Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan.
Department of Nutrition, Chung Shan Medical University, No.110, Sec.1, Jiangou N. Rd., South Dist., Taichung City, 40201, Taiwan.
Plant Foods Hum Nutr. 2024 Jun;79(2):374-380. doi: 10.1007/s11130-024-01192-9. Epub 2024 May 15.
Desmodium caudatum extracts (DCE) were investigated for their potential therapeutic effects on diabetic nephropathy (DN). In our study, the high-fat diet (HFD) / streptozotocin (STZ)-induced DN model in C57BL/6 mice was treated with 100 mg/kg, 200 mg/kg DCE. The results showed that DCE decreased biochemical parameters and proteinuria levels. The kidney sections staining indicated that DCE treatment recovered glomerular atrophy and alleviated lipid droplets in the glomerular. Additionally, DCE inhibited lipid and glycogen accumulation down-regulated the expression of sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS) proteins. DCE also reduced collagenous fibrous tissue and the expression of transforming growth factor-β1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) through Masson's trichrome staining and immunohistochemical analysis. We found that DCE alleviated hydroxyproline content, and epithelial-mesenchymal transition (EMT). Besides, the results shown that DCE enhanced the antioxidant enzymes to mitigate fibrosis by reducing oxidative stress. In conclusion, our study provided evidence of the protective effect of DCE which down-regulated hyperglycemia, hyperlipidemia and inhibition of TGF-β1 and EMT pathway but elevated antioxidant, suggesting its therapeutic implication for DN.
落花生属植物提取物（DCE）被研究其对糖尿病肾病（DN）的潜在治疗作用。在我们的研究中，用 100mg/kg、200mg/kg 的 DCE 处理高脂肪饮食（HFD）/链脲佐菌素（STZ）诱导的 C57BL/6 小鼠 DN 模型。结果表明，DCE 降低了生化参数和蛋白尿水平。肾脏切片染色表明，DCE 治疗恢复了肾小球萎缩，减轻了肾小球中的脂质滴。此外，DCE 通过抑制脂质和糖原积累，下调固醇调节元件结合蛋白 1（SREBP1）和脂肪酸合酶（FAS）蛋白的表达。DCE 还通过 Masson 三色染色和免疫组织化学分析减少胶原纤维组织和转化生长因子-β1（TGF-β1）和α-平滑肌肌动蛋白（α-SMA）的表达。我们发现 DCE 通过减轻氧化应激来减轻羟脯氨酸含量和上皮-间充质转化（EMT）。此外，结果表明，DCE 通过增强抗氧化酶来减轻纤维化，从而抑制 TGF-β1 和 EMT 途径，但提高了抗氧化能力，表明其对 DN 的治疗意义。
