1α,25-二羟基维生素D3抑制商陆有丝分裂原刺激的人B细胞活化：在无血清培养条件下的分析
1 alpha,25-dihydroxyvitamin D3 inhibits pokeweed mitogen-stimulated human B-cell activation: an analysis using serum-free culture conditions.
作者信息
Shiozawa K, Shiozawa S, Shimizu S, Fujita T
出版信息
Immunology. 1985 Sep;56(1):161-7.
The effect of 1 alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on pokeweed mitogen-induced generation of human immunoglobulin-secreting cells (ISC) was studied in serum-free culture. Human peripheral blood mononuclear cells (1.75 X 10(6)/ml) were cultured in 200 microliter microculture wells containing Iscove's MEM supplemented with 20 micrograms/ml of human transferrin, 20 micrograms/ml of soybean lecithin and 1 mg/ml of delipidated bovine albumin. The culture supported ISC generation of all isotypes, and the optimal concentration of pokeweed mitogen was 1 microgram/ml. After stimulation with a suboptimal concentration (0.3 micrograms/ml) of pokeweed mitogen, 1,25-(OH)2D3 suppressed the ISC generation at a dose range between 10(-9) M and 10(-7) M, and maximal inhibition was obtained at 10(-7) M. 1,25-(OH)2D3 had no effect on the spontaneous ISC generation. The effect was specific for 1,25-(OH)2D3 and no suppression was obtained by 24R,25-dihydroxyvitamin D3 or 25-hydroxyvitamin D3.
在无血清培养中研究了1α,25 - 二羟基维生素D3（1,25-(OH)2D3）对商陆丝裂原诱导的人免疫球蛋白分泌细胞（ISC）生成的影响。将人外周血单个核细胞（1.75×10⁶/ml）培养于含有伊思柯夫改良的伊格尔培养基（Iscove's MEM）的200微升微孔培养孔中，该培养基补充有20微克/毫升的人转铁蛋白、20微克/毫升的大豆卵磷脂和1毫克/毫升的脱脂牛血清白蛋白。该培养体系支持所有同种型ISC的生成，商陆丝裂原的最佳浓度为1微克/毫升。用次优浓度（0.3微克/毫升）的商陆丝裂原刺激后，1,25-(OH)2D3在10⁻⁹M至10⁻⁷M的剂量范围内抑制ISC的生成，在10⁻⁷M时获得最大抑制作用。1,25-(OH)2D3对自发的ISC生成无影响。该作用对1,25-(OH)2D3具有特异性，24R,25 - 二羟基维生素D3或25 - 羟基维生素D3未产生抑制作用。
Zotero 插件