Kabadayı Şahin Esra, Şenat Almila, Söğüt İbrahim, Duymaz Tomris, Erel Özcan
Department of Psychiatry, Ankara Yıldırım Beyazıt University, Faculty of Medicine, Ankara, Turkey.
Department of Biochemistry, Istanbul Taksim Training and Research Hospital, Istanbul, Turkey.
Psychiatry Clin Psychopharmacol. 2023 Sep 1;33(3):170-176. doi: 10.5152/pcp.2023.23636. eCollection 2023 Sep.
This study aimed to evaluate oxidative damage by measuring erythrocytic reduced/oxidized glutathione as an intracellular thiol pool and serum thiol/disulfide homeostasis as an extracellular thiol pool in patients with opioid use disorder.
In this prospective cross-sectional study, 33 male patients diagnosed with opioid use disorder and 30 healthy male controls were included. Sociodemographic characteristics and psychometric analyzes were performed and addiction characteristics (duration and amount of heroin use, usage methods) were recorded. For the evaluation of oxidative balance, intracellular reduced-oxidized glutathione (reduced glutathione and oxidized glutathione), and extracellular thiol-disulfide (native thiol and disulfide) levels were measured.
There was a decrease in reduced glutathione and native thiol levels and an increase in GSSG and SS levels. Similarly, while oxidized/reduced glutathione, oxidized/total glutathione%, and disulfide/native thiol % ratios increased, the ratio of reduced glutathione/total glutathione% and native thiol/total thiol% decreased. Moreover, a positive correlation was found between the level of both intracellular and extracellular oxidant molecules and the duration and amount of opioid use.
Impaired intracellular reduced glutathione/oxidized glutathione and extracellular disulfide/native thiol homeostasis were found in patients with opioid use disorder. The intracellular and extracellular oxidative stress may cause complications related to chronic opioid use.
本研究旨在通过测量红细胞中还原型/氧化型谷胱甘肽作为细胞内硫醇池以及血清硫醇/二硫键稳态作为细胞外硫醇池，来评估阿片类药物使用障碍患者的氧化损伤。
在这项前瞻性横断面研究中，纳入了33名被诊断为阿片类药物使用障碍的男性患者和30名健康男性对照。进行了社会人口学特征和心理测量分析，并记录了成瘾特征（海洛因使用的持续时间和量、使用方法）。为评估氧化平衡，测量了细胞内还原型-氧化型谷胱甘肽（还原型谷胱甘肽和氧化型谷胱甘肽）以及细胞外硫醇-二硫键（天然硫醇和二硫键）水平。
还原型谷胱甘肽和天然硫醇水平降低，而谷胱甘肽二硫化物（GSSG）和二硫键（SS）水平升高。同样，虽然氧化型/还原型谷胱甘肽、氧化型/总谷胱甘肽百分比以及二硫键/天然硫醇百分比比值增加，但还原型谷胱甘肽/总谷胱甘肽百分比和天然硫醇/总硫醇百分比比值降低。此外，发现细胞内和细胞外氧化剂分子水平与阿片类药物使用的持续时间和量之间存在正相关。
在阿片类药物使用障碍患者中发现细胞内还原型谷胱甘肽/氧化型谷胱甘肽以及细胞外二硫键/天然硫醇稳态受损。细胞内和细胞外氧化应激可能导致与慢性阿片类药物使用相关的并发症。
