橙皮素负载氧化石墨烯纳米片（Hsp-GO）作为结肠癌药物传递系统的比较评价：合成与抗癌效率评估。
Comparative evaluation of hesperetin-loaded graphene oxide nanosheets (Hsp-GO) as a drug delivery system for colon cancer: synthesis and anticancer efficiency assessment.
机构信息
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
出版信息
Mol Biol Rep. 2024 Apr 29;51(1):591. doi: 10.1007/s11033-024-09504-7.
BACKGROUND
Graphene oxide nanosheets (GONS) are recognized for their role in enhancing drug delivery and effectiveness in cancer treatment. With colon cancer being a prevalent global issue and the significant side effects associated with chemotherapy, the primary treatment for colon cancer alongside surgery, there is a critical need for novel therapeutic strategies to support patients in combating this disease. Hesperetin (HSP), a natural compound found in specific fruits, exhibits anti-cancer properties. The aim of this study is to investigate the effect of GONS on the LS174t colon cancer cell line.
METHODS
In this study, an anti-cancer nano-drug was synthesized by creating a hesperetin-graphene oxide nanocomposite (Hsp-GO), which was subsequently evaluated for its efficacy through in vitro cell toxicity assays. Three systems were investigated: HSP, GONS, and HSP-loaded GONS, to determine their cytotoxic and pro-apoptotic impacts on the LS174t colon cancer cell line, along with assessing the expression of BAX and BCL2. The morphology and properties of both GO and Hsp-GO were examined using scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared spectroscopy (FTIR).
RESULTS
The Hsp-GO nanocomposite displayed potent cytotoxic and pro-apoptotic effects on LS174t colon cancer cells, outperforming individual treatments with HSP or GONS. Cell viability assays showed a significant decrease in cell viability with Hsp-GO treatment. Analysis of BAX and BCL2 expression revealed elevated BAX and reduced BCL2 levels in Hsp-GO treated cells, indicating enhanced apoptotic activity. Morphological analysis confirmed successful Hsp-GO synthesis, while structural integrity was supported by X-ray diffraction and FTIR analyses.
CONCLUSIONS
These study highlight the potential of Hsp-GO as a promising anti-cancer nano-drug for colon cancer therapy.
背景
氧化石墨烯纳米片（GONS）因其在增强药物递送和癌症治疗效果方面的作用而受到关注。由于结肠癌是一个普遍存在的全球问题，并且化疗存在显著的副作用，作为结肠癌的主要治疗方法，除了手术之外，还需要新的治疗策略来支持患者对抗这种疾病。橙皮苷（HSP）是一种存在于某些水果中的天然化合物，具有抗癌特性。本研究旨在探讨 GONS 对 LS174t 结肠癌细胞系的影响。
方法
本研究通过构建橙皮苷-氧化石墨烯纳米复合物（Hsp-GO）合成了一种抗癌纳米药物，随后通过体外细胞毒性试验评估其疗效。研究了三种系统：HSP、GONS 和负载 HSP 的 GONS，以确定它们对 LS174t 结肠癌细胞系的细胞毒性和促凋亡作用，并评估 BAX 和 BCL2 的表达。使用扫描电子显微镜（SEM）、X 射线衍射和傅里叶变换红外光谱（FTIR）对 GO 和 Hsp-GO 的形貌和性质进行了检测。
结果
Hsp-GO 纳米复合材料对 LS174t 结肠癌细胞具有强大的细胞毒性和促凋亡作用，优于 HSP 或 GONS 的单独治疗。细胞活力测定显示 Hsp-GO 处理后细胞活力显著下降。BAX 和 BCL2 表达分析显示，Hsp-GO 处理细胞中 BAX 升高，BCL2 降低，表明凋亡活性增强。形态分析证实了 Hsp-GO 的成功合成，而 X 射线衍射和 FTIR 分析则支持其结构完整性。
结论
这些研究结果强调了 Hsp-GO 作为结肠癌治疗有前途的抗癌纳米药物的潜力。
Zotero 插件