Clutterbuck R D, Hills C A, Hoey P, Alexander P, Powles R L, Millar J L
Leuk Res. 1985;9(12):1511-8. doi: 10.1016/0145-2126(85)90044-x.
Human AML cells from the blood of a series of patients have been implanted subcutaneously into mice immune-suppressed by thymectomy and total-body irradiation. Solid tumours resulted from 18 out of 19 samples and their growth was compared with the proliferation of AML cells in culture. In 17 cases tumours grew to a maximum size and then spontaneously regressed. Cells from one patient produced tumours which did not regress and could be retransplanted into freshly immune-suppressed mice. Cells from a human promyelocytic cell line (HL60) also produced nonregressing and retransplantantable tumours. Normal human mononuclear bone marrow cells implanted s.c. produced a growth pattern similar to that of the majority of AML cells. A second inoculum of AML cells into animals with regressing tumours also produced tumours and thus regression cannot be accounted for on the basis of returning immunity. AML cells placed into short-term suspension culture invariably matured to monocyte/macrophage type cells and/or granulocytic cells as identified by cytochemical staining. However, no correlation was observed between proliferation or maturation of cells in culture, and tumour growth in vivo. Cells derived from disaggregated AML tumours also showed evidence of myeloid differentiation suggesting that tumour regression is due to maturation of leukaemic cells.
从一系列患者血液中获取的人类急性髓系白血病（AML）细胞已被皮下植入经胸腺切除和全身照射免疫抑制的小鼠体内。19个样本中有18个形成了实体瘤，并将其生长情况与培养中的AML细胞增殖情况进行了比较。在17例中，肿瘤生长到最大尺寸后自发消退。一名患者的细胞产生的肿瘤未消退，且可重新移植到新的免疫抑制小鼠体内。来自人类早幼粒细胞系（HL60）的细胞也产生了不消退且可重新移植的肿瘤。皮下植入的正常人单核骨髓细胞产生的生长模式与大多数AML细胞相似。将AML细胞再次接种到肿瘤正在消退的动物体内也会产生肿瘤，因此肿瘤消退不能归因于恢复的免疫力。通过细胞化学染色鉴定，置于短期悬浮培养中的AML细胞总是成熟为单核细胞/巨噬细胞类型的细胞和/或粒细胞。然而，未观察到培养中细胞的增殖或成熟与体内肿瘤生长之间存在相关性。来自解离的AML肿瘤的细胞也显示出髓系分化的证据，这表明肿瘤消退是由于白血病细胞的成熟。
