Palú G, Selby P, Powles R, Alexander P
Br J Cancer. 1979 Nov;40(5):731-5. doi: 10.1038/bjc.1979.253.
A population of human AML cells which have a characteristic karyotypic marker was cryopreserved and then grown in short-term liquid culture for 2 weeks, during which time the cells increased about 7-fold in number and progressively acquired characteristics of macrophages. 10(7) cells obtained after 1 day in culture, when they were almost devoid of Fc receptors (Fc-), on inoculation into immune-deprived mice gave rise to tumours in more than 90% of the animals. However, after 13 days of culture, when almost all the cells had Fc receptors (Fc+), a similar inoculum did not grow as tumours. After 7 days in culture the cells were heterogeneous, and divided about equally into Fc+ and Fc- cells, both of which were replicating. The Fc- population was capable of producing tumours, whereas the Fc+ was not. Of 23 assessable xenograft tumours produced by the AML cells, 14 regressed completely, 4 grew progressively and 5 grew progressively after initial regression. Progressive tumours could be further transplanted. The regression may arise as a result of maturation in vivo similar to that seen in vitro.
一群具有特征性核型标记的人类急性髓系白血病（AML）细胞被冷冻保存，然后在短期液体培养中培养2周，在此期间细胞数量增加了约7倍，并逐渐获得巨噬细胞的特征。培养1天后获得的10⁷个细胞，此时它们几乎没有Fc受体（Fc-），接种到免疫缺陷小鼠体内后，超过90%的动物产生了肿瘤。然而，培养13天后，当几乎所有细胞都有Fc受体（Fc+）时，同样的接种物并未长成肿瘤。培养7天后，细胞是异质性的，大约平均分为Fc+和Fc-细胞，两者都在复制。Fc-群体能够产生肿瘤，而Fc+群体则不能。由AML细胞产生的23个可评估的异种移植肿瘤中，14个完全消退，4个逐渐生长，5个在最初消退后逐渐生长。进展性肿瘤可以进一步移植。消退可能是由于体内成熟类似于体外所见的结果。
