University of Kashmir.
Section of Plant Pathology, Mycology and Microbiology, Department of Botany, University of Kashmir, Srinagar-190006, J&K, India.
Int J Med Mushrooms. 2024;26(6):39-51. doi: 10.1615/IntJMedMushrooms.2024053250.
In the current era, wild macrofungi are being focused for developing and overing novel bioactive compounds for the management of agricultural, horticultural, and other infectious diseases. In that view, current research work was designed to evaluate the biochemical composition and medicinal properties of Morchella crassipes mushroom. The mycochemical screening of aqueous extract exposed the incidence of glycosides, free amino acids and proteins, alkaloids, carbs, flavonoids, terpenoids, phenolic compounds and tannins, except volatile oils, resins, steroids, and anthraquinones. However, hexane extract exhibited the occurrence of glycosides, alkaloids, volatile oils, steroids and terpenoids while as all other phytochemicals were not detected. The gas chromatography mass spectrometry profiling has disclosed the identification of three predominant naturally occurring bioactive volatile monoterpenoids, namely neral, citral, and epoxy-linalool oxide with well-known biological activities. The methanolic extract resulted in strong antifungal efficacy against the tested fungal strains such as Penicillium chrysogenum (20.33 ± 0.57 mm) followed by Pythium ultimum (15.33 ± 0.76 mm) and Aspergillus niger (12.50 ± 0.50 mm) at highest concentrations. Likewise, marked antibacterial effects were reported in case of Staphylococcus aureus (15.16 ± 0.76 mm), followed by Salmonella gallinarum (14.33 ± 0.57 mm) and Escherichia coli (13.66 ± 0.57 mm), respectively. This data may offer baseline information regarding the bioactive metabolites and opening new ways for conducting trails to find natural management strategies to combat multi drug resistant pathogens in horticulture, agriculture, and aquaculture.
在当前时代，人们专注于开发野生大型真菌，以寻找用于防治农业、园艺和其他传染病的新型生物活性化合物。基于这一观点，目前的研究旨在评估羊肚菌的生化成分和药用特性。水提物的微生物化学筛选揭示了糖苷、游离氨基酸和蛋白质、生物碱、碳水化合物、类黄酮、萜类、酚类化合物和单宁的存在，而挥发油、树脂、甾体和蒽醌除外。然而，正己烷提取物则显示出糖苷、生物碱、挥发油、甾体和萜类的存在，而其他所有植物化学物质则未被检测到。气相色谱-质谱联用分析揭示了三种主要的天然生物活性挥发性单萜的鉴定，即柠檬醛、香茅醛和环氧芳樟醇氧化物，它们具有众所周知的生物活性。甲醇提取物对测试真菌菌株具有很强的抗真菌功效，如青霉素（20.33±0.57mm）、腐霉（15.33±0.76mm）和黑曲霉（12.50±0.50mm），在最高浓度下。同样，金黄色葡萄球菌（15.16±0.76mm）、鸡白痢沙门氏菌（14.33±0.57mm）和大肠杆菌（13.66±0.57mm）也表现出明显的抗菌作用。这些数据可能为生物活性代谢物提供了基础信息，并为开展试验以寻找天然管理策略来对抗园艺、农业和水产养殖中的多药耐药病原体提供了新的途径。
