Rhimi Wafa, Ben Salem Issam, Immediato Davide, Saidi Mouldi, Boulila Abdennacer, Cafarchia Claudia
Faculté des Sciences de Bizerte, Université de Carthage, 7021 Zarzouna, Tunisia.
Laboratory of Biotechnology and Nuclear Technolog, National Centre of Nuclear Science and Technology (CNSTN), Sidi Thabet Technopark, 2020 Ariana, Tunisia.
Molecules. 2017 Jun 30;22(7):942. doi: 10.3390/molecules22070942.
The small amount of data regarding the antifungal activity of (L.) Greuter against dermatophytes, spp. and spp., associated with the few comparative studies on the antimicrobial activity of methanolic, ethanolic, and butanolic extracts underpins the study herein presented. The total condensed tannin (TCT), phenol (TPC), flavonoid (TFC), and caffeoylquinic acid (CQC) content of methanol, butanol, and ethanol (80% and 100%) extracts of were assessed and their bactericidal and fungicidal activities were evaluated. The antibacterial, anti- and anti- activities were evaluated by using the disk diffusion method, whereas the anti- and anti- activities were assessed by studying the toxicity effect of the extracts on vegetative growth, sporulation and germination. The methanolic extract contained the highest TPC and CQC content. It contains several phytochemicals mainly caffeoylquinic acid derivatives as determined by liquid chromatography with photodiode array and electrospray ionisation mass spectrometric detection (LC/PDA/ESI-MS) analysis. All extracts showed an excellent inhibitory effect against bacteria and spp., whereas methanolic extract exhibited the highest antifungal activities against spp. and strains. The results clearly showed that all extracts, in particular the methanolic extract, might be excellent antimicrobial drugs for treating infections that are life threatening (i.e., ) or infections that require mandatory treatments (i.e., or ).
关于（L.）Greuter对皮肤癣菌、 属和 属的抗真菌活性的少量数据，以及对甲醇、乙醇和丁醇提取物抗菌活性的少数比较研究，为本文所呈现的研究提供了依据。对 的甲醇、丁醇和乙醇（80%和100%）提取物中的总缩合单宁（TCT）、酚类（TPC）、黄酮类（TFC）和咖啡酰奎尼酸（CQC）含量进行了评估，并评估了它们的杀菌和杀真菌活性。采用纸片扩散法评估抗菌、抗 和抗 活性，而通过研究提取物对营养生长、孢子形成和萌发的毒性作用来评估抗 和抗 活性。甲醇提取物的TPC和CQC含量最高。通过液相色谱-光电二极管阵列和电喷雾电离质谱检测（LC/PDA/ESI-MS）分析确定，它含有几种植物化学物质，主要是咖啡酰奎尼酸衍生物。所有提取物对细菌和 属均表现出优异的抑制作用，而甲醇提取物对 属和 菌株表现出最高的抗真菌活性。结果清楚地表明，所有提取物，特别是甲醇提取物，可能是治疗危及生命的感染（即 ）或需要强制治疗的感染（即 或 ）的优异抗菌药物。
