Department of Animal and Poultry Science, Faculty of Agricultural Technology, College of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.
Department of Animal and Poultry Science, Faculty of Agricultural Technology, College of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.
Poult Sci. 2024 Jul;103(7):103842. doi: 10.1016/j.psj.2024.103842. Epub 2024 May 12.
Aging leads to decreased fertility in roosters, which is likely due to increased oxidative stress. This study evaluated the antioxidant effects of gallic acid (GA) supplementation on sperm quality and fertility of aged roosters. This study evaluated whether GA supplementation can mitigate age-related fertility decline. Roosters were randomly assigned to: control, 100 mg/kg GA, or 200 mg/kg GA. Semen parameters, sperm kinetics, hormone levels, fertility rate, and hatchability were assessed. GA increased semen concentration, membrane integrity and viability while decreasing defects versus control (P < 0.01). Testosterone was higher in GA groups (P<0.01) without affecting gonadotropins. Furthermore, 200 mg/kg GA optimized motility, velocity, linearity, and beat cross frequency versus control and 100 mg/kg GA (P < 0.01). Fertility and hatchability were higher in both GA groups. In conclusion, GA supplementation in aged roosters improves sperm quality, antioxidant status, testosterone, and fertility outcomes, likely by mitigating oxidative stress. The 200 mg/kg dose elicited optimal effects on motion parameters.
衰老大龄导致公鸡繁殖力下降，这可能是由于氧化应激增加所致。本研究评估了没食子酸（GA）补充剂对老龄公鸡精子质量和生育力的抗氧化作用。本研究评估了 GA 补充剂是否可以减轻与年龄相关的生育力下降。公鸡被随机分配到以下三组：对照组、100mg/kg GA 组和 200mg/kg GA 组。评估了精液参数、精子动力学、激素水平、受精率和孵化率。与对照组相比，GA 增加了精液浓度、膜完整性和活力，而降低了缺陷率（P<0.01）。GA 组的睾丸激素水平更高（P<0.01），而不影响促性腺激素。此外，与对照组和 100mg/kg GA 组相比，200mg/kg GA 优化了运动性、速度、直线性和鞭打频率（P<0.01）。两个 GA 组的受精率和孵化率都更高。综上所述，GA 补充剂可改善老龄公鸡的精子质量、抗氧化状态、睾丸激素和生育力，可能是通过减轻氧化应激。200mg/kg 的剂量对运动参数产生了最佳效果。
