De Broe M E, Roels F, Nouwen E J, Claeys L, Wieme R J
Hepatology. 1985 Jan-Feb;5(1):118-28. doi: 10.1002/hep.1840050124.
This study presents biochemical, histochemical, morphological and immunological evidence that part of the high molecular weight alkaline phosphatase observed in the serum of patients with liver disease and particularly in cases of intrahepatic cholestasis or focal-, extrahepatic obstruction originates from the liver plasma membrane. The high molecular weight protein alkaline phosphatase complex contains several plasma membrane enzymes and behaves like a plasma membrane fragment after isopycnic density gradient ultracentrifugation in sucrose, cesium chloride and metrizamide. Electron microscopic examination revealed a triple-layered vesicle which retained alkaline phosphatase activity. Incubation of human liver cells with anti-serum against purified high molecular weight multienzyme complex resulted in fixation of antibodies on the plasma membrane as shown by positive plasma membrane fluorescence. These plasma membrane fragments in the serum are not of biliary origin.
本研究提供了生化、组织化学、形态学和免疫学证据，表明在肝病患者血清中观察到的部分高分子量碱性磷酸酶，特别是在肝内胆汁淤积或局灶性、肝外梗阻病例中，起源于肝细胞膜。高分子量蛋白质碱性磷酸酶复合物包含几种细胞膜酶，在蔗糖、氯化铯和甲泛葡胺中进行等密度梯度超速离心后，其行为类似于细胞膜片段。电子显微镜检查发现了一种保留碱性磷酸酶活性的三层囊泡。用针对纯化的高分子量多酶复合物的抗血清孵育人肝细胞，如阳性细胞膜荧光所示，导致抗体固定在细胞膜上。血清中的这些细胞膜片段并非来自胆汁。
