Li Yuntong, Li Jiaqing, Li Songshan, Xu Zhengjie, Ma Wei, Wu Xinyan, Yan Yayi, Wang Ying, Hu Andina
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Guangzhou, China.
Heliyon. 2024 May 10;10(10):e31023. doi: 10.1016/j.heliyon.2024.e31023. eCollection 2024 May 30.
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a life-threatening disease with largely unknown intraocular pathogenesis. Herein, we determined the presence of SARS-CoV-2-specific ribonucleic acid (RNA) and virus-associated antibodies in the vitreous humor of people who have recently recovered from SARS-CoV-2 infection.
This cross-sectional study included 33 patients (33 eyes) who have recently recovered from SARS-CoV-2 infection. Vitreous humor and blood serum samples were tested for the SARS-CoV-2 RNA and virus-associated antibodies.
Among 33 participants, blood serum and vitreous humor were all tested negative for SARS-CoV-2 RNA. SARS-CoV-2-specific IgM was detected in 87.88 % (29/33) patients in blood serum and 6.10 % (2/33) in vitreous humor; SARS-CoV-2-specific IgG was detected in 96.97 % (32/33) patient in blood serum and 81.82 % (27/33) in vitreous humor. Statistical significance was found for IgM expression between blood serum and vitreous humor ( < 0.01), while IgG was not ( = 0.11). The days after recovery were statistically longer both in IgM-positive blood serum samples group and IgG-positive vitreous humor samples group compared with negative samples of each group ( < 0.01). Additionally, no statistical difference could be detected in antibody expression in vitreous humor between different groups divided on the condition of the risk of blood-retina-barrier (BRB) failure ( = 0.49 for IgM; = 0.37 for IgG).
After recovering from COVID-19, no SARS-CoV-2 RNA was detected in vitreous humor, but anti-CoV-2 IgM was detected in 6.1 % and IgG in approximately 80 % of vitreous humor samples of participants. We also found that the positivety rate of SARS-CoV-2-specific antibodies in the blood serum and vitreous humor were both correlated with the days after recovery since the infection.
2019冠状病毒病（COVID-19）由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起，是一种危及生命的疾病，其眼内发病机制 largely unknown。在此，我们确定了近期从SARS-CoV-2感染中康复的患者玻璃体液中SARS-CoV-2特异性核糖核酸（RNA）和病毒相关抗体的存在情况。
这项横断面研究纳入了33例近期从SARS-CoV-2感染中康复的患者（33只眼）。对玻璃体液和血清样本进行SARS-CoV-2 RNA和病毒相关抗体检测。
33名参与者中，血清和玻璃体液的SARS-CoV-2 RNA检测均为阴性。血清中87.88%（29/33）的患者和玻璃体液中6.10%（2/33）的患者检测到SARS-CoV-2特异性IgM；血清中96.97%（32/33）的患者和玻璃体液中81.82%（27/33）的患者检测到SARS-CoV-2特异性IgG。血清和玻璃体液中IgM表达存在统计学意义（<0.01），而IgG无统计学意义（=0.11）。与每组阴性样本相比，IgM阳性血清样本组和IgG阳性玻璃体液样本组康复后天数在统计学上更长（<0.01）。此外，根据血视网膜屏障（BRB）破坏风险划分的不同组之间，玻璃体液中抗体表达无统计学差异（IgM为0.49；IgG为0.37）。
COVID-19康复后，玻璃体液中未检测到SARS-CoV-2 RNA，但参与者约6.1%的玻璃体液样本中检测到抗-CoV-2 IgM，约80%检测到IgG。我们还发现，血清和玻璃体液中SARS-CoV-2特异性抗体的阳性率均与感染后康复天数相关。
