酵母染色体复制子的分离与鉴定

Isolation and characterisation of a yeast chromosomal replicator.

作者信息

Stinchcomb D T, Struhl K, Davis R W

出版信息

Nature. 1979 Nov 1;282(5734):39-43. doi: 10.1038/282039a0.

DOI:10.1038/282039a0

Abstract

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.

摘要

一个表现为染色体复制子的酵母DNA序列，即ars1（自主复制序列）已被分离出来。在转化过程中，ars1能使所有共线性DNA自主复制。该复制子可整合到其他复制单元中，并能以多聚体形式发挥作用。这个850碱基对的ars1元件与其他酵母序列没有可检测到的同源性。这种含复制子的质粒可用于在酵母细胞中分离DNA序列以及研究染色体DNA复制。