School of Physics, Universiti Sains Malaysia, 11800, Gelugor, Penang, Malaysia.
Eman Biodiscoveries Sdn Bhd, A1-4, Lot 5, Persiaran 2/1, Kedah Halal Park, Sungai Petani Industrial Area, 08000, Sungai Petani, Kedah, Malaysia.
Lasers Med Sci. 2024 Jun 1;39(1):147. doi: 10.1007/s10103-024-04102-2.
Photobiomodulation (PBM) holds promise as a therapy modality, but its applicability is hindered by the lack of a quantitative model to predict the optimal dose for all forms of PBM. This study investigated the optimal PBM parameters for 532 nm green laser irradiation on SHSY5Y neuroblastoma cells, a commonly used in vitro model for neurodegenerative disease studies. A two-tailed, two sample t-test with equal variance was used to obtain the p-values and statistical significance. There are 3 sets of parameters showing significant positive percentage biostimulation. produce a percentage biostimulation of ; produce a percentage biostimulation of ; and produce a percentage biostimulation of . The highest significant percentage bioinhibition observed is for (dose: ) producing a bioinhibition of . After identifying several parameters that produce noticeable photobiological effects (biostimulation and bioinhibition), this study compared the reaction of undifferentiated and differentiated SHSY5Y cells to laser irradiation and found that undifferentiated SHSY5Y cells shows greater photobiological effect from 532 nm laser irradiation . This study demonstrated the differentiation-dependant photobiological effect of SHSY5Y in 532 nm laser PBM. This shows that considerations on the differentiation state of cells is important in PBM studies. The hypothesis of difference in intracellular reactive oxygen species (ROS) accumulation from laser irradiation can serve as a versatile explanation of the observed difference in photobiological effect. Further investigation into the role of ROS as a mediator of various photobiological effects from laser of different wavelengths is warranted.
光生物调节（PBM）作为一种治疗方式具有很大的应用潜力，但其适用性受到缺乏定量模型来预测所有形式的 PBM 的最佳剂量的限制。本研究调查了 532nm 绿光激光照射 SHSY5Y 神经母细胞瘤细胞的最佳 PBM 参数，该细胞是神经退行性疾病研究中常用的体外模型。使用具有相等方差的双侧、两样本 t 检验来获得 p 值和统计学意义。有 3 组参数显示出显著的正百分比生物刺激。参数 1 产生的百分比生物刺激为 ；参数 2 产生的百分比生物刺激为 ；参数 3 产生的百分比生物刺激为 。观察到的最高显著百分比生物抑制是对于 （剂量： ），产生生物抑制 。在确定了几个产生明显光生物效应（生物刺激和生物抑制）的参数后，本研究比较了未分化和分化的 SHSY5Y 细胞对激光照射的反应，发现未分化的 SHSY5Y 细胞对 532nm 激光照射表现出更大的光生物效应 。本研究证明了 SHSY5Y 在 532nm 激光 PBM 中的分化依赖性光生物效应。这表明在 PBM 研究中考虑细胞的分化状态是很重要的。激光照射导致细胞内活性氧（ROS）积累差异的假设可以作为观察到的光生物效应差异的通用解释。进一步研究不同波长激光的 ROS 作为各种光生物效应的介质的作用是有必要的。
