School of Health and Life Sciences, University of the West of Scotland, High St, Paisley, PA1 2BE, UK.
School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Bearsden Rd, Glasgow, G61 1QH, UK.
BMC Vet Res. 2024 Jun 1;20(1):236. doi: 10.1186/s12917-024-04062-3.
The chicken's inflammatory response is an essential part of the bird's response to infection. A single dose of Escherichia coli (E. coli) lipopolysaccharide (LPS) endotoxin can activate the acute phase response (APR) and lead to the production of acute phase proteins (APPs). In this study, the responses of established chicken APPs, Serum amyloid A (SAA) and Alpha-1-acid-glycoprotein (AGP), were compared to two novel APPs, Hemopexin (Hpx) and Extracellular fatty acid binding protein (Ex-FABP), in 15-day old broilers over a time course of 48 h post E.coli LPS challenge. We aimed to investigate and validate their role as biomarkers of an APR. Novel plant extracts, Citrus (CTS) and cucumber (CMB), were used as dietary supplements to investigate their ability to reduce the inflammatory response initiated by the endotoxin.
A significant increase of established (SAA, AGP) and novel (Ex-FABP, Hpx) APPs was detected post E.coli LPS challenge. Extracellular fatty acid binding protein (Ex-FABP) showed a similar early response to SAA post LPS challenge by increasing ~ 20-fold at 12 h post challenge (P < 0.001). Hemopexin (Hpx) showed a later response by increasing ∼5-fold at 24 h post challenge (P < 0.001) with a similar trend to AGP. No differences in APP responses were identified between diets (CTS and CMB) using any of the established or novel biomarkers.
Hpx and Ex-FABP were confirmed as potential biomarkers of APR in broilers when using an E. coli LPS model along with SAA and AGP. However, no clear advantage for using either of dietary supplements to modulate the APR was identified at the dosage used.
鸡的炎症反应是鸟类对感染的反应的重要组成部分。单次给予大肠杆菌（E. coli）脂多糖（LPS）内毒素可激活急性期反应（APR），并导致急性期蛋白（APP）的产生。在这项研究中，在大肠杆菌 LPS 挑战后 48 小时的时间过程中，比较了已建立的鸡 APPs，血清淀粉样蛋白 A（SAA）和α-1-酸性糖蛋白（AGP），以及两种新的 APPs，血红素结合蛋白（Hpx）和细胞外脂肪酸结合蛋白（Ex-FABP）在 15 日龄肉鸡中的反应。我们旨在研究并验证它们作为 APR 生物标志物的作用。新型植物提取物，柑橘（CTS）和黄瓜（CMB），被用作膳食补充剂，以研究它们减少内毒素引发的炎症反应的能力。
在大肠杆菌 LPS 挑战后，检测到已建立的（SAA、AGP）和新的（Ex-FABP、Hpx）APPs 的显著增加。细胞外脂肪酸结合蛋白（Ex-FABP）在 LPS 挑战后 12 小时内表现出与 SAA 相似的早期反应，增加了约 20 倍（P<0.001）。血红素结合蛋白（Hpx）在 24 小时后反应较慢，增加了约 5 倍（P<0.001），与 AGP 的趋势相似。使用任何已建立或新的生物标志物，在饮食（CTS 和 CMB）之间均未发现 APP 反应的差异。
当使用大肠杆菌 LPS 模型时，Hpx 和 Ex-FABP 与 SAA 和 AGP 一起被确认为 APR 的潜在生物标志物。然而，在使用的剂量下，没有明显的优势可以使用任何膳食补充剂来调节 APR。
