van der Groen G, Beelaert G
J Virol Methods. 1985 Jan;10(1):53-8. doi: 10.1016/0166-0934(85)90088-6.
A technique, using indirect immunoperoxidase antibody (IPA), was developed for the detection of IgG antibody to Hantaan virus. The same protein A-peroxidase conjugate was used with mouse, rat and human sera. The IPA technique employs glass slides with air-dried gamma-ray-inactivated and acetone-fixed Hantaan-infected Vero-E6 cells. Antibody titers detected by IPA was comparable to those detected by the indirect fluorescent antibody technique.
一种使用间接免疫过氧化物酶抗体（IPA）的技术被开发用于检测抗汉坦病毒的IgG抗体。相同的蛋白A-过氧化物酶结合物用于小鼠、大鼠和人血清。IPA技术采用载有经γ射线灭活并经丙酮固定的感染汉坦病毒的Vero-E6细胞的空气干燥玻片。通过IPA检测的抗体滴度与通过间接荧光抗体技术检测的滴度相当。
