Department of Food Science and Human Nutrition, University of Illinois Urbana-Champaign, Urbana, IL, United States.
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, IL, United States.
J Nutr. 2024 Aug;154(8):2396-2410. doi: 10.1016/j.tjnut.2024.05.028. Epub 2024 Jun 1.
Honey improves probiotic survival in vitro. However, if this effect translates to humans has not been investigated.
We aimed to determine effects of honey plus yogurt containing the probiotic Bifidobacterium animalis subsp. lactis DN-173 010/CNCM I-2494 (B. animalis) on intestinal transit time, probiotic enrichment, digestive health, mood, and cognition in adults.
Sixty-six healthy adults (34 female; 33.6 ± 9.8 y; 24.6 ± 3.0 kg/m) in a crossover trial were randomly assigned to 2-wk yogurt conditions in a counterbalanced order with ≥4-wk washout: 1) Honey (HON): yogurt plus honey and 2) Negative Control (NC): heat-treated yogurt plus sugar. Of the participants, n = 62 completed the trial, and n = 37 (17 female; 32.0 ± 8.3 y; 25.0 ± 2.9 kg/m) elected to enroll in a third condition (a nonrandomized study extension) after ≥4-wk washout with a reference Positive Control (PC): yogurt plus sugar. At baseline and end of each of the 3 conditions, intestinal transit time was measured with dye capsules; probiotic abundance with fecal DNA 16S sequencing; digestive health with symptom/function records, Bristol stool consistency, Gastrointestinal Tolerability, and Gastrointestinal Quality of Life Index; mood with Positive and Negative Affect Schedule-Short Form, Depression Anxiety Stress Scales-42, Patient-Reported Outcomes Measurement Information System questionnaires, and an emotional image task; and cognition with a spatial reconstruction task. Data were analyzed using linear mixed-effects models (LMMs) with significance at P ≤ 0.05. Baseline and end data were included in the LMM, with fixed effects being treatment, time, treatment by time interaction, and baseline covariate, and the random effect being the participant.
B. animalis was enriched in HON (d = 3.54; P = 0.0002) compared to controls with linear discriminant analysis effect size. Intestinal transit time, gastrointestinal health, mood, and cognition did not differ between conditions (LMM: Ps > 0.05).
Yogurt + honey enriched B. animalis but did not reduce intestinal transit time or have other functional gastrointestinal, mood, or cognitive effects in adults. This trial was registered at www.
gov as NCT04187950 and NCT04901390.
蜂蜜可提高益生菌在体外的存活率。然而，这种效果是否能转化为人类还尚未可知。
本研究旨在确定蜂蜜加含有益生菌双歧杆菌乳亚种 DN-173 010/CNCM I-2494（B. animalis）的酸奶对成年人的肠道转运时间、益生菌丰度、消化健康、情绪和认知的影响。
在一项交叉试验中，我们随机分配 66 名健康成年人（34 名女性；33.6 ± 9.8 岁；24.6 ± 3.0 kg/m），按平衡顺序进行为期 2 周的酸奶处理，洗脱期≥4 周：1）蜂蜜（HON）：酸奶加蜂蜜，2）阴性对照（NC）：热处理酸奶加糖。其中 62 名参与者完成了试验，37 名（17 名女性；32.0 ± 8.3 岁；25.0 ± 2.9 kg/m）在洗脱期≥4 周后选择参加第三个条件（非随机研究扩展），采用阳性对照（PC）：酸奶加糖。在基线和每个条件的结束时，我们使用染色胶囊测量肠道转运时间；使用粪便 DNA 16S 测序测量益生菌丰度；使用症状/功能记录、布里斯托粪便稠度、胃肠道耐受性和胃肠道生活质量指数测量消化健康；使用积极和消极情绪量表-短格式、抑郁焦虑应激量表-42、患者报告的结果测量信息系统问卷和情绪图像任务测量情绪；使用空间重建任务测量认知。采用线性混合效应模型（LMMs）进行数据分析，显著性水平为 P≤0.05。LMM 中包括基线和结束时的数据，固定效应为处理、时间、处理与时间的交互作用和基线协变量，随机效应为参与者。
与对照组相比，HON 中 B. animalis 的丰度增加（d = 3.54；P = 0.0002），差异具有线性判别分析效应量。肠道转运时间、胃肠道健康、情绪和认知在各组之间无差异（LMM：P＞0.05）。
酸奶加蜂蜜可增加 B. animalis 的丰度，但不会降低成年人的肠道转运时间或对其胃肠道、情绪或认知功能产生其他影响。本试验已在 www.clinicaltrials.gov 上注册，注册号为 NCT04187950 和 NCT04901390。
