Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Republic of Korea.
Korean Collection for Type Cultures (KCTC) Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Dec;73(12). doi: 10.1099/ijsem.0.006193.
A Gram-stain-negative, aerobic, non-flagellated and coccoid or ovoid bacterial strain, BSSL-BM11, was isolated from sand of coastal dunes along the Yellow Sea of the Korean peninsula. Strain BSSL-BM11 grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences, the up-to-date bacterial core gene set and average amino acid identity (AAI) showed that strain BSSL-BM11 forms a cluster with the type strains of and . Strain BSSL-BM11 showed 16S rRNA gene sequence similarities of 98.3 and 98.0 % to the type strains of and , respectively, and less than 96.4 % to the type strains of the other recognized species. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain BSSL-BM11 and the type strains of and were 77.0-84.8 % and 20.0-28.1 %, respectively. The 16S rRNA gene similarity, AAI, ANI and dDDH values between Z8 and KCTC 42249 were 98.0, 77.4, 76.7 and 20.1 %, respectively. The DNA G+C content of strain BSSL-BM11 from genomic sequence data was 61.3 mol%. Strain BSSL-BM11 contained Q-10 as the predominant ubiquinone and C 7, C and cyclo C 8 as the major fatty acids. The major polar lipids of strain BSSL-BM11 were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Based on the polyphasic data, it is proposed that be reclassified as a member of the genus . Phenotypic and phylogenetic analyses revealed that strain BSSL-BM11 is separated from and . On the basis of the data presented here, strain BSSL-BM11 (=KACC 21634=NBRC 114503) is considered to represent a novel species of the genus , for which the name sp. nov. is proposed.
一株革兰氏阴性、需氧、无鞭毛、球形或卵形的细菌菌株 BSSL-BM11 是从朝鲜半岛黄海沿岸沙丘的沙中分离得到的。菌株 BSSL-BM11 在 30°C、pH7.0-8.0 和 2.0-3.0%（w/v）NaCl 存在的条件下生长最佳。基于 16S rRNA 基因序列、最新的细菌核心基因集和平均氨基酸同一性（AAI）构建的系统发育树显示，菌株 BSSL-BM11 与 和 的模式菌株形成一个聚类。菌株 BSSL-BM11 与 和 模式菌株的 16S rRNA 基因序列相似性分别为 98.3%和 98.0%，与其他公认种的模式菌株的相似性均低于 96.4%。菌株 BSSL-BM11 与 和 模式菌株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 77.0-84.8%和 20.0-28.1%。Z8 和 KCTC 42249 之间的 16S rRNA 基因相似性、AAI、ANI 和 dDDH 值分别为 98.0%、77.4%、76.7%和 20.1%。基于基因组序列数据，菌株 BSSL-BM11 的 DNA G+C 含量为 61.3mol%。菌株 BSSL-BM11 含有 Q-10 作为主要的泛醌，C 7、C 和环 C 8 作为主要的脂肪酸。菌株 BSSL-BM11 的主要极性脂是磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。基于多相数据，建议将 重新分类为属的一个成员。表型和系统发育分析表明，菌株 BSSL-BM11 与 和 分离。基于这里呈现的数据，菌株 BSSL-BM11（=KACC 21634=NBRC 114503）被认为代表属的一个新种，提议将其命名为 sp. nov.。
