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酵母染色质被脱氧核糖核酸酶I均匀消化。

Yeast chromatin is uniformly digested by DNase-I.

作者信息

Lohr D, Hereford L

出版信息

Proc Natl Acad Sci U S A. 1979 Sep;76(9):4285-8. doi: 10.1073/pnas.76.9.4285.

Abstract

The DNase I (EC 3.1.21.1) sensitivity of transcribed yeast chromatin has been examined. We find that, in contrast to chromatin from higher eukaryotes, transcribed yeast chromatin and total yeast chromatin are equally sensitive to DNase I digestion. We interpret these results to mean that the entire yeast genome exists in a state that represents a restricted proportion of total chromatin in higher eukaryotes.

摘要

已对转录的酵母染色质的脱氧核糖核酸酶I(EC 3.1.21.1)敏感性进行了检测。我们发现,与高等真核生物的染色质不同,转录的酵母染色质和总酵母染色质对脱氧核糖核酸酶I消化的敏感性相同。我们将这些结果解释为意味着整个酵母基因组以一种代表高等真核生物总染色质受限比例的状态存在。

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本文引用的文献

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SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
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Analysis of DNA of isolated chromatin subunits.分离染色质亚基的DNA分析
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Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8. doi: 10.1073/pnas.72.3.1184.

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