Lohr D, Ide G
Nucleic Acids Res. 1979;6(5):1909-27. doi: 10.1093/nar/6.5.1909.
We have compared the structure of intra-nuclear and isolated chromatin from logarithmically growing yeast cells to chromatin from cells which had entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern, 160 bp repeat size, with staphylococcal nuclease and similar variability in repeat sizes within the genome. DNase I produces the same ladder (less than 120 b) and a quite similar extended ladder (120-300 b) which shows that both chromatins have phased nucleosomes. However, the rate of DNase I digestion of growing phase is greater than in stationary. Functionally speaking, growing phase nuclei are 5-20 times as active in the rate of endogenous transcription (all three polymerases are involved). The transcriptional and DNase I susceptibility differences noted in nuclei are maintained in sucrose gradient isolated oligonucleosomes and mononucleosomes from the two states.
我们比较了对数生长期酵母细胞的核内染色质和分离染色质的结构，以及进入稳定期并停止生长的细胞的染色质结构。两种染色质均显示出相似的核小体重复模式，重复大小为160 bp，用葡萄球菌核酸酶处理后，基因组内重复大小的变异性相似。DNase I产生相同的梯状条带（小于120 bp）和非常相似的扩展梯状条带（120 - 300 bp），这表明两种染色质都有相位核小体。然而，生长期DNase I的消化速率大于稳定期。从功能上讲，生长期细胞核的内源性转录速率（涉及所有三种聚合酶）是稳定期的5 - 20倍。在细胞核中观察到的转录和DNase I敏感性差异，在从这两种状态通过蔗糖梯度分离得到的寡核小体和单核小体中依然存在。
