Majster Mirjam, Almer Sven, Malmqvist Sebastian, Johannsen Annsofi, Lira-Junior Ronaldo, Boström Elisabeth Almer
Division of Oral Diagnostics and Rehabilitation, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.
Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Oral Dis. 2025 Jan;31(1):286-297. doi: 10.1111/odi.15036. Epub 2024 Jun 9.
Calprotectin is elevated in saliva from inflammatory bowel disease (IBD) patients, but it is also affected by oral disease. We assessed the salivary concentration of calprotectin in IBD patients, in relation to intestinal and oral diseases. Furthermore, we investigated the phenotype of salivary neutrophils from IBD patients, and their ability to secrete calprotectin.
Thirty IBD patients and 26 controls were orally examined and sampled for stimulated saliva. Twenty-five IBD patients provided fresh fecal samples. Calprotectin concentrations in saliva and feces were determined by an enzyme-linked immunosorbent assay. Expression of CD11b, CD15, and CD16 on oral neutrophils was assessed by flow cytometry. Secretion of calprotectin was evaluated in cultured oral neutrophils.
Calprotectin was significantly elevated in saliva of IBD patients compared to controls, particularly in Crohn's disease, irrespective of caries or periodontitis. Salivary calprotectin did not correlate to fecal calprotectin. CD11b expression was significantly reduced in salivary neutrophils from IBD patients. Salivary neutrophils from IBD patients tended to secrete more calprotectin than controls.
Salivary calprotectin is elevated in IBD regardless of oral diseases. Furthermore, salivary neutrophils secrete calprotectin, and display lower CD11b expression in IBD.
钙卫蛋白在炎症性肠病（IBD）患者的唾液中升高，但它也受口腔疾病影响。我们评估了IBD患者唾液中钙卫蛋白的浓度，并将其与肠道和口腔疾病相关联。此外，我们研究了IBD患者唾液中性粒细胞的表型及其分泌钙卫蛋白的能力。
对30例IBD患者和26例对照者进行口腔检查，并采集刺激性唾液样本。25例IBD患者提供新鲜粪便样本。采用酶联免疫吸附测定法测定唾液和粪便中钙卫蛋白的浓度。通过流式细胞术评估口腔中性粒细胞上CD11b、CD15和CD16的表达。在培养的口腔中性粒细胞中评估钙卫蛋白的分泌情况。
与对照组相比，IBD患者唾液中的钙卫蛋白显著升高，尤其是在克罗恩病患者中，无论是否患有龋齿或牙周炎。唾液钙卫蛋白与粪便钙卫蛋白无相关性。IBD患者唾液中性粒细胞中CD11b的表达显著降低。IBD患者唾液中性粒细胞分泌的钙卫蛋白往往比对照组更多。
无论口腔疾病如何，IBD患者唾液中的钙卫蛋白都会升高。此外，唾液中性粒细胞分泌钙卫蛋白，并在IBD中表现出较低的CD11b表达。
