Gosney J R, Sissons M C
Thorax. 1985 Mar;40(3):194-8. doi: 10.1136/thx.40.3.194.
The nature and distribution of bronchopulmonary endocrine cells immunoreactive for calcitonin was studied in normal adult Wistar albino rats by immunoenzyme histochemistry with the peroxidase-antiperoxidase technique. A widespread distribution of both solitary endocrine cells and neuroepithelial bodies immunoreactive for calcitonin was found. Many, but not all, were also immunoreactive for neurone specific enolase. Although both classes of cells were present in airways and parenchyma, most of the solitary cells were found in alveolar ducts and alveoli whereas most of the neuroepithelial bodies were located in bronchi and bronchioles. Bronchopulmonary endocrine cells are generally regarded as being sparse in the adult rat. It is suggested that this may be a consequence of the use of inadequate methods in attempting to identify them. So far as is known, this is the first time that calcitonin has been demonstrated by immunohistochemical methods in the lungs of rats.
采用过氧化物酶-抗过氧化物酶技术的免疫酶组织化学方法，对成年正常Wistar白化大鼠中降钙素免疫反应性支气管肺内分泌细胞的性质和分布进行了研究。发现了降钙素免疫反应性单个内分泌细胞和神经上皮小体的广泛分布。许多（但不是全部）细胞对神经元特异性烯醇化酶也有免疫反应性。虽然这两类细胞都存在于气道和实质中，但大多数单个细胞见于肺泡管和肺泡，而大多数神经上皮小体位于支气管和细支气管。支气管肺内分泌细胞在成年大鼠中通常被认为是稀少的。有人认为，这可能是在试图识别它们时使用了不充分方法的结果。据所知，这是首次通过免疫组织化学方法在大鼠肺中证实降钙素的存在。
