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针对鹦鹉热衣原体免疫荧光检测的鸡胚检测比较效价。

Comparative titers of egg assay against immunofluorescent assay of Chlamydia psittaci.

作者信息

Tessler J

出版信息

Can J Comp Med. 1985 Jan;49(1):109-11.

PMID:3886106
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1236128/
Abstract

A comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in Vero cells with and without cortisone plus cytochalasin B. The titer of NJ-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin B and cortisone. The SCT and CDC strains had approximately the same titers in the egg titration and the fluorescent antibody assay for samples with and without the antimetabolites.

摘要

对在添加和不添加可的松加细胞松弛素B的情况下于Vero细胞中繁殖的三种衣原体菌株,进行了在鸡胚试验和直接荧光抗体试验之间的滴度比较。NJ - 1菌株在未处理样品中的鸡胚滴定和荧光抗体试验中的滴度相似,而在用细胞松弛素B和可的松处理的样品中滴度略低。对于添加和未添加抗代谢物的样品,SCT和CDC菌株在鸡胚滴定和荧光抗体试验中的滴度大致相同。

相似文献

1
Comparative titers of egg assay against immunofluorescent assay of Chlamydia psittaci.针对鹦鹉热衣原体免疫荧光检测的鸡胚检测比较效价。
Can J Comp Med. 1985 Jan;49(1):109-11.
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本文引用的文献

1
QUANTITATIVE ASSAY OF PSITTACOSIS VIRUS BY THE FLUORESCENT CELL-COUNTING TECHNIQUE.用荧光细胞计数技术对鹦鹉热病毒进行定量测定。
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The titration of trachoma and inclusion blennorrhoea viruses in cell cultures.沙眼病毒和包涵体性脓漏眼病毒在细胞培养中的滴定
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The infected cell count method of titration of feline pneumonitis virus.猫肺炎病毒滴定的感染细胞计数法。
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4
Effects of cortisone, cytochalasin B and cycloheximide on strains of Chlamydia psittaci in cell cultures.可的松、细胞松弛素B和放线菌酮对细胞培养中鹦鹉热衣原体菌株的影响。
Can J Comp Med. 1983 Jul;47(3):352-7.
5
Immunofluorescence plaque assay for African swine fever virus.非洲猪瘟病毒免疫荧光蚀斑试验
Can J Comp Med. 1974 Oct;38(4):443-7.
6
An epornitic of fatal chlamydiosis (ornithosis) in South Carolina turkeys.
J Am Vet Med Assoc. 1975 Jan 15;166(2):175-8.
7
Infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated McCoy cells.豚鼠包涵体结膜炎病原体在经辐照的 McCoy 细胞中的感染性滴定
J Gen Microbiol. 1976 Aug;96(2):249-56. doi: 10.1099/00221287-95-2-249.
8
Biotyping of Chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide.基于包涵体形态以及对二乙氨基乙基葡聚糖和放线菌酮的反应对鹦鹉热衣原体进行生物分型。
Infect Immun. 1979 Apr;24(1):224-32. doi: 10.1128/iai.24.1.224-232.1979.
9
Chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells.
J Infect Dis. 1979 Dec;140(6):959-67. doi: 10.1093/infdis/140.6.959.