Gard A L, White F P, Dutton G R
J Neuroimmunol. 1985 Jun;8(4-6):359-75. doi: 10.1016/s0165-5728(85)80073-4.
The dendritic processes and perinuclear cytoplasm of stellate-shaped perisinusoidal cells in frozen sections of rat liver were specifically labeled with antisera raised independently to glial fibrillary acidic protein (GFAP), the major component of intermediate filaments in astrocytes. A liver protein co-migrating with authentic GFAP and immunoreactive with GFAP antisera was demonstrated with immunoblots of brain and liver extracts enriched in intermediate filament proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study presents yet another example of immunoreactivity to GFAP, or a highly similar protein localized outside the CNS, in cells of mesenchymal origin exhibiting some morphological features common to astroglia.
在大鼠肝脏冰冻切片中，肝血窦周呈星状的细胞的树突状突起和核周细胞质被分别用针对神经胶质纤维酸性蛋白（GFAP）产生的抗血清特异性标记，GFAP是星形胶质细胞中间丝的主要成分。通过对富含中间丝蛋白的脑和肝提取物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离后进行免疫印迹分析，证实了一种与真实GFAP共迁移且能与GFAP抗血清发生免疫反应的肝脏蛋白。本研究展示了另一个对GFAP或一种高度相似的位于中枢神经系统之外的蛋白产生免疫反应的例子，这种反应出现在具有一些星形胶质细胞共有的形态学特征的间充质来源细胞中。
