Kriechbaum Ricarda, Spadiut Oliver, Kopp Julian
Research Division: Biochemical Engineering, Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorferstraße 1a, 1060 Wien, Austria.
Microorganisms. 2024 Jun 18;12(6):1222. doi: 10.3390/microorganisms12061222.
Lignocellulosic biomass is abundant on Earth, and there are multiple acidic pretreatment options to separate the cellulose, hemicellulose, and lignin fraction. By doing so, the fermentation inhibitors 5-Hydroxymethylfurfural (HMF) and furfural (FF) are produced in varying concentrations depending on the hydrolyzed substrate. In this study, the impact of these furanic compounds on growth and photosynthetic activity was analyzed. Both compounds led to a prolonged lag phase in growth. While the photosynthetic yield Y(II) was not significantly influenced in cultivations containing HMF, FF significantly reduced Y(II). The conversion of 5-Hydroxymethylfurfural and furfural to 5-Hydroxymethyl-2-Furoic Acid and 2-Furoic Acid was observed. In total, 100% of HMF and FF was converted in photoautotrophic and mixotrophic cultivations. The results demonstrate that is, as of now, the first known microalgal species converting furanic compounds.
木质纤维素生物质在地球上储量丰富，有多种酸性预处理方法可分离纤维素、半纤维素和木质素部分。通过这种方式，会产生浓度各异的发酵抑制剂5-羟甲基糠醛（HMF）和糠醛（FF），其浓度取决于水解的底物。在本研究中，分析了这些呋喃类化合物对生长和光合活性的影响。这两种化合物均导致生长的延迟期延长。虽然在含有HMF的培养物中光合产量Y(II)未受到显著影响，但FF显著降低了Y(II)。观察到5-羟甲基糠醛和糠醛向5-羟甲基-2-糠酸和2-糠酸的转化。在光自养和混合营养培养中，HMF和FF总共100%被转化。结果表明，截至目前，这是首个已知的能转化呋喃类化合物的微藻物种。
