用于检测尿道和宫颈拭子中沙眼衣原体抗原的酶免疫测定法。
Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs.
作者信息
Mumtaz G, Mellars B J, Ridgway G L, Oriel J D
出版信息
J Clin Pathol. 1985 Jul;38(7):740-2. doi: 10.1136/jcp.38.7.740.
Abstract
An enzyme immunoassay technique based on the direct detection of Chlamydia trachomatis antigen in urethral or cervical swabs was used for the rapid diagnosis of chlamydial genital infection. Urethral and cervical samples from 140 patients were tested in parallel by enzyme immunoassay and cell culture using iodine staining. The direct test had a sensitivity of 92.5% and specificity of 97.2% when compared with the cell culture system. The enzyme immunoassay technique provides a rapid and simple method for diagnosing chlamydial genital infection and may be performed on a large number of samples in laboratories which do not have tissue culture facilities or a trained microscopist.
摘要
一种基于直接检测尿道或宫颈拭子中沙眼衣原体抗原的酶免疫测定技术被用于衣原体性生殖器感染的快速诊断。对140例患者的尿道和宫颈样本同时采用酶免疫测定法和使用碘染色的细胞培养法进行检测。与细胞培养系统相比，直接检测法的灵敏度为92.5%，特异性为97.2%。酶免疫测定技术为诊断衣原体性生殖器感染提供了一种快速简便的方法，并且可以在没有组织培养设施或训练有素的显微镜检查人员的实验室中对大量样本进行检测。
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本文引用的文献
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J Clin Microbiol. 1984 Sep;20(3):465-7. doi: 10.1128/jcm.20.3.465-467.1984.
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Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies.使用单克隆抗体对沙眼衣原体进行非培养诊断。
N Engl J Med. 1984 May 3;310(18):1146-50. doi: 10.1056/NEJM198405033101803.
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Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation.使用荧光素标记单克隆抗体检测涂片沙眼衣原体原体的敏感性：与传统衣原体分离法的比较
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J Clin Microbiol. 1977 Oct;6(4):328-31. doi: 10.1128/jcm.6.4.328-331.1977.
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