School of Physics, University of St Andrews, St Andrews, UK.
Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.
Methods Mol Biol. 2024;2833:109-119. doi: 10.1007/978-1-0716-3981-8_11.
Tuberculosis (TB) is the most common cause of death from an infectious disease. Although treatment has been available for more than 70 years, it still takes too long and many patients default risking relapse and the emergence of resistance. It is known that lipid-rich, phenotypically antibiotic-tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse necessitating extended therapy. Using a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis we can perform optical analysis in the form of wavelength-modulated Raman spectroscopy (WMRS) on the trapped organisms. This system can allow observations of the mycobacteria for up to 8 h. By adding antibiotics, it is possible to study the effect of antibiotics in real-time by comparing the Raman fingerprints in comparison to the unstressed condition. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to many conditions including antibiotic stress, and changes in the growth media. This opens the possibility of understanding better the stimuli that trigger the lipid-rich downregulated and phenotypically antibiotic-resistant cell state.
结核病（TB）是最常见的传染病死因。尽管治疗方法已经存在 70 多年，但它仍然需要太长时间，许多患者违约，冒着复发和出现耐药性的风险。已知富含脂质、表型抗生素耐受的细菌对抗生素的抵抗力更强，可能是导致需要延长治疗的复发的原因。我们使用一种微流控系统，通过声学捕获活分枝杆菌，即结核分枝杆菌的模型生物，我们可以对被困生物进行波长调制拉曼光谱（WMRS）的光学分析。该系统可以允许对分枝杆菌进行长达 8 小时的观察。通过添加抗生素，可以通过与未受应激条件下的拉曼指纹进行比较，实时研究抗生素的作用。这个微流控平台可以用来研究任何微生物，并动态监测其对许多条件的反应，包括抗生素应激和生长介质的变化。这为更好地了解触发富含脂质和表型抗生素耐药细胞状态的刺激因素提供了可能性。
