Afriyie Daniel Kwame, Ameyaw Elvis Ofori, Henneh Isaac Tabiri, Asare George, Ofori-Atta Ebenezer, Amponsah Seth Kwabena, Appiah-Opong Regina
Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana.
Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana.
J Toxicol. 2024 Mar 7;2024:7526701. doi: 10.1155/2024/7526701. eCollection 2024.
Mull. Arg. is a traditional medicinal plant frequently employed in Ghana for the treatment of benign prostatic hyperplasia and prostate cancer. The objective of this study was to determine the acute oral toxicity of the aqueous stem extract of (CMASE) in male Sprague-Dawley (S-D) rats. The acute toxicity of CMASE was evaluated using S-D rats randomly divided into four groups of five animals each. Three groups (low dose, median dose, and high dose) of rats received single oral doses of CMASE (1000, 2500, and 5000 mg/kg body weight, respectively) using an oral gavage. The control group was given distilled water. After 14 days of daily observations, hematological, biochemical, and histopathological analyses were conducted on the rats. From the results obtained, doses of CMASE up to 5000 mg/kg did not cause death or induce any clinical indications of toxicity during the study period. Also, the mean body weight and the hematological indices assessed were not significantly affected by the various doses of CMASE compared to the control group. However, serum uric acid and creatinine levels decreased significantly ( < 0.001) 14 days after the extract administration. Serum liver function enzyme levels, including alkaline phosphatase (ALP), alanine aminotransferases (ALT), and aspartate aminotransferases (AST), and serum proteins (total proteins and albumin) exhibited significant ( < 0.001) non dose-dependent changes (increases and decreases) in treated groups compared to the controls. Other biochemical indices, however, did not differ significantly between the treated groups and the controls. The gross pathological and histological analysis of the heart, liver, and kidney tissues did not reveal any significant changes in histoarchitecture. The oral LD of CMASE in rats was greater than 5000 mg/kg, indicating that the extract was relatively safe. It must, however, be used with care as a substitute for the roots.
穆勒氏银胶菊是加纳一种常用于治疗良性前列腺增生和前列腺癌的传统药用植物。本研究的目的是确定穆勒氏银胶菊水提茎提取物（CMASE）对雄性斯普拉格-道利（S-D）大鼠的急性经口毒性。使用随机分为四组、每组五只动物的S-D大鼠评估CMASE的急性毒性。三组大鼠（低剂量、中剂量和高剂量组）通过灌胃分别接受单次口服剂量的CMASE（分别为1000、2500和5000毫克/千克体重）。对照组给予蒸馏水。经过14天的每日观察后，对大鼠进行血液学、生化和组织病理学分析。从获得的结果来看，在研究期间，高达5000毫克/千克剂量的CMASE未导致死亡或诱发任何毒性临床迹象。此外，与对照组相比，不同剂量的CMASE对评估的平均体重和血液学指标没有显著影响。然而，提取物给药14天后，血清尿酸和肌酐水平显著降低（P<0.001）。与对照组相比，治疗组血清肝功能酶水平，包括碱性磷酸酶（ALP）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）以及血清蛋白（总蛋白和白蛋白）呈现显著（P<0.001）的非剂量依赖性变化（升高和降低）。然而，其他生化指标在治疗组和对照组之间没有显著差异。心脏、肝脏和肾脏组织的大体病理和组织学分析未发现组织结构有任何显著变化。CMASE在大鼠中的经口半数致死量大于5000毫克/千克，表明该提取物相对安全。然而，作为根的替代品使用时必须谨慎。
