一种分泌具有特定特异性的猪单克隆抗体的猪-鼠杂交瘤。
A porcine-murine hybridoma that secretes porcine monoclonal antibody of defined specificity.
作者信息
Raybould T J, Willson P J, McDougall L J, Watts T C
出版信息
Am J Vet Res. 1985 Aug;46(8):1768-9.
PMID:3898933
Abstract
Spleen cells from a pig hyperimmunized with Escherichia coli were fused with nonproducer mouse plasmacytoma cells. Stable hybridoma lines secreting porcine immunoglobulins were obtained. One line secreted monoclonal porcine immunoglobulin G, which reacted specifically with E coli in an enzyme-linked immunosorbent assay and precipitated a polypeptide of molecular weight of 50,000. This is the first report of a stable porcine-murine hybridoma producing porcine antibody of defined specificity.
摘要
用大肠杆菌对猪进行超免疫后获取的脾细胞，与不产生抗体的小鼠浆细胞瘤细胞进行融合。获得了分泌猪免疫球蛋白的稳定杂交瘤细胞系。其中一个细胞系分泌单克隆猪免疫球蛋白G，在酶联免疫吸附测定中它能与大肠杆菌发生特异性反应，并沉淀出一种分子量为50,000的多肽。这是关于产生具有特定特异性猪抗体的稳定猪 - 鼠杂交瘤的首次报道。
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