补充益生菌对双氯芬酸诱导的肝毒性大鼠氧化应激标志物的影响。
Effect of probiotic supplementation on oxidative stress markers in rats with diclofenac-induced hepatotoxicity.
机构信息
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University Mohamed Seddik Benyahia of Jijel, Jijel, Algeria.
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
出版信息
Braz J Microbiol. 2020 Dec;51(4):1615-1622. doi: 10.1007/s42770-020-00302-4. Epub 2020 May 27.
Abstract
In this study, we have investigated the effect of an antioxidant probiotic pretreatment toward an overdose of diclofenac in rats (100 mg/kg bw). Rats were treated daily with the probiotic Streptococcus salivarius St.sa (10 CFU) during seven successive days and then received a single treatment with diclofenac overdose in distilled water. Liver transaminases (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase), histology, glutathione (GSH) and malondialdehyde (MDA) level were investigated. In addition, both antioxidant enzyme activity and its mRNA gene expression were studied to evaluate diclofenac hepatotoxicity. The results indicated that probiotic pretreatment reduced diclofenac-induced hepatotoxicity through enhancement of the studied hepatic markers and regulation of antioxidant enzyme expression and activity. These findings indicate that the probiotic pretreatment protects rat liver against the oxidative stress induced by diclofenac overdose.
摘要
在这项研究中，我们研究了抗氧化益生菌预处理对大鼠过量服用双氯芬酸（100mg/kg bw）的影响。大鼠在连续 7 天内每天接受唾液链球菌 St.sa（10CFU）的益生菌治疗，然后用蒸馏水单次给予过量的双氯芬酸。研究了肝转氨酶（丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶）、组织学、谷胱甘肽（GSH）和丙二醛（MDA）水平。此外，还研究了抗氧化酶活性及其 mRNA 基因表达，以评估双氯芬酸的肝毒性。结果表明，益生菌预处理通过增强研究的肝标志物以及调节抗氧化酶的表达和活性，减轻了双氯芬酸引起的肝毒性。这些发现表明，益生菌预处理可保护大鼠肝脏免受双氯芬酸过量引起的氧化应激。
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