直接光谱法测定亚铁螯合酶活性
Direct Spectroscopic Ferrochelatase Assay.
机构信息
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Department of Microbiology, University of Georgia, Athens, GA, USA.
出版信息
Methods Mol Biol. 2024;2839:243-247. doi: 10.1007/978-1-0716-4043-2_14.
Ferrochelatases (E.C. 4.99.1.1) catalyze the insertion of ferrous iron into either protoporphyrin IX to make protoheme IX or coproporphyrin III to make coproheme III. Ferrochelatase activity in extracts or purified protein can be measured via several assays. Here, we describe a rapid real-time direct spectroscopic ferrochelatase assay for both protoporphyrin and coproporphyrin ferrochelatases.
亚铁螯合酶（E.C. 4.99.1.1）催化亚铁离子插入原卟啉 IX 中生成原血红素 IX 或粪卟啉 III 生成粪卟啉 III。可通过几种测定法来测量提取物或纯化蛋白中的亚铁螯合酶活性。本文描述了一种用于原卟啉和粪卟啉亚铁螯合酶的快速实时直接光谱法亚铁螯合酶测定法。
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