Gheorghiu Mihaela
Biosensors Department, International Centre of Biodynamics, Bucharest 060101, Romania.
J Biomed Res. 2020 Dec 25;35(4):255-263. doi: 10.7555/JBR.34.20200128.
Current cell-based biosensors have progressed substantially from mere alternatives to molecular bioreceptors into enabling tools for interfacing molecular machineries and gene circuits with microelectronics and for developing groundbreaking sensing and theragnostic platforms. The recent literature concerning whole-cell biosensors is reviewed with an emphasis on mammalian cells, and the challenges and breakthroughs brought along in biomedical analyses through novel biosensing concepts and the synthetic biology toolbox. These recent innovations allow development of cell-based biosensing platforms having tailored performances and capable to reach the levels of sensitivity, dynamic range, and stability suitable for high analytic/medical relevance. They also pave the way for the construction of flexible biosensing platforms with utility across biological research and clinical applications. The work is intended to stimulate interest in generation of cell-based biosensors and improve their acceptance and exploitation.
当前基于细胞的生物传感器已经从单纯的分子生物受体替代品取得了实质性进展，成为连接分子机器和基因电路与微电子学的 enabling 工具，以及用于开发开创性传感和治疗诊断平台的工具。本文综述了近期关于全细胞生物传感器的文献，重点关注哺乳动物细胞，以及通过新型生物传感概念和合成生物学工具箱在生物医学分析中带来的挑战和突破。这些最新创新使得能够开发出具有定制性能的基于细胞的生物传感平台，并能够达到适用于高分析/医学相关性的灵敏度、动态范围和稳定性水平。它们还为构建适用于生物研究和临床应用的灵活生物传感平台铺平了道路。这项工作旨在激发人们对基于细胞的生物传感器的兴趣，并提高它们的接受度和利用率。
