Aguilar Paola, Fonseca Angélica Cristina, Garlet Gustavo Pompermaier, Gulinelli Jéssica Lemos, Santos Pâmela Letícia
Universidade de Araraquara - Department of Health Sciences - Dental School - Araraquara (SP) - Brazil.
Universidade de São Paulo - Department of Biological Science - São Paulo (SP) - Brazil.
Acta Cir Bras. 2024 Jul 15;39:e393724. doi: 10.1590/acb393724. eCollection 2024.
To evaluate collagen fibers during the bone repair process in critical defects created in the tibias of rats, treated with zoledronic acid (AZ) associated with low-level laser therapy (LLLT).
Ten rats were distributed according to treatment: group 1) saline solution; group 2) LLLT; group 3) AZ; group 4) AZ and LLLT. AZ was administered at the dose of 0.035 mg/kg at fortnightly intervals over eight weeks. Next, 2-mm bone defects were created in the tibias of all animals. The bone defects in groups 2 and 4 were irradiated LLLT in the immediate postoperative period. After periods 14 and 28 of application, the animals were euthanized, and birefringence analysis was performed.
Approximately 90% of the total area was occupied by collagen fibers within the red color spectrum, this area being statistically larger in relation to the area occupied by collagen fibers within the green and yellow spectrum, in the four groups. Over the 14-day period, there was no statistically significant difference between the groups. In the 28-day period, group 2 (14.02 ± 15.9%) was superior in quantifying green birefringent fibers compared to group 1 (3.06 ± 3.24%), with p = 0.009.
LLLT associated with ZA is effective in stimulating the neoformation of collagen fibers. The LLLT group without the association with ZA showed a greater amount of immature and less organized matrix over a period of 28 days.
评估唑来膦酸（AZ）联合低强度激光治疗（LLLT）对大鼠胫骨临界性骨缺损修复过程中胶原纤维的影响。
将10只大鼠按治疗方法分组：第1组）生理盐水；第2组）LLLT；第3组）AZ；第4组）AZ和LLLT。在8周内，每两周以0.035mg/kg的剂量给予AZ。接下来，在所有动物的胫骨上制造2mm的骨缺损。第2组和第4组的骨缺损在术后即刻接受LLLT照射。在应用14天和28天后，对动物实施安乐死并进行双折射分析。
在四组中，红色光谱内的胶原纤维占据了总面积的约90%，该区域在统计学上相对于绿色和黄色光谱内胶原纤维所占区域更大。在14天期间，各组之间无统计学显著差异。在28天期间，与第1组（3.06±3.24%）相比，第2组（14.02±15.9%）在绿色双折射纤维定量方面更优，p = 0.009。
ZA联合LLLT可有效刺激胶原纤维的新生。未联合ZA的LLLT组在28天内显示出更多的未成熟且排列较无序的基质。
