Department of Microbiology, School of Medicine, Shahid Beheshti University of Medial Science, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sci Rep. 2024 Jul 19;14(1):16712. doi: 10.1038/s41598-024-67677-4.
The association between oral dysbiosis and celiac disease (CD) remains poorly understood, as does the impact of CD-associated dysbiosis on disease development or exacerbation. This study aims to investigate alterations in salivary microbial composition among children with CD. In this cross-sectional study, saliva samples from 12 children with active CD (A-CD group), 14 children with CD on a gluten-free diet (GFD), and 10 healthy control (HC) children were analyzed using DNA sequencing targeting the 16S ribosomal RNA. Both patients in A-CD and GFD groups showed a significant increase (p = 0.0001) in the Bacteroidetes phylum, while the Actinobacteria phylum showed a significant decrease (p = 0.0001). Notably, the Rothia genus and R.aeria also demonstrated a significant decrease (p = 0.0001) within the both CD groups as compare to HC. Additionally, the control group displayed a significant increase (p = 0.006) in R.mucilaginosa species compared to both CD patient groups. Distinct bacterial strains were abundant in the saliva of patients with active CD, indicating a unique composition of the salivary microbiome in individuals with CD. These findings suggest that our approach to assessing salivary microbiota changes may contribute to developing noninvasive methods for diagnosing and treating CD.
口腔菌群失调与乳糜泻（CD）之间的关联尚未得到充分理解，CD 相关的菌群失调对疾病发展或恶化的影响也是如此。本研究旨在调查 CD 患儿唾液微生物组成的变化。在这项横断面研究中，对 12 名活动性 CD（A-CD 组）患儿、14 名接受无麸质饮食（GFD）治疗的 CD 患儿和 10 名健康对照（HC）儿童的唾液样本进行了分析，采用靶向 16S 核糖体 RNA 的 DNA 测序。A-CD 和 GFD 两组患者的拟杆菌门均显著增加（p=0.0001），而放线菌门则显著减少（p=0.0001）。值得注意的是，与 HC 相比，罗斯氏菌属和 R.aeria 也在两个 CD 组中显著减少（p=0.0001）。此外，与 CD 患者组相比，对照组的 R.mucilaginosa 种显著增加（p=0.006）。在活动性 CD 患者的唾液中存在丰富的独特细菌菌株，表明 CD 患者唾液微生物组具有独特的组成。这些发现表明，我们评估唾液微生物群变化的方法可能有助于开发用于诊断和治疗 CD 的非侵入性方法。
