Hu Sheng, Wang Yangcheng, Wang Yanzhe, Chen Xiaoming, Tong Ruijie
College of Information Science and Engineering, Northeastern University, Shenyang, China; Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Qinhuangdao, 066004, China.
College of Information Science and Engineering, Northeastern University, Shenyang, China.
J Chromatogr A. 2024 Aug 30;1731:465155. doi: 10.1016/j.chroma.2024.465155. Epub 2024 Jul 9.
It is indispensable to realize the high level of purification and separation, so that objective particles, such as malignant cells, harmful bacteria, and special proteins or biological molecules, could satisfy the high precise measurement in the pharmaceutical analysis, clinical diagnosis, targeted therapy, and food defense. In addition, this could reveal the intrinsic nature and evolution mechanisms of individual biological variations. Consequently, many techniques related to optical tweezers, microfluidics, acoustophoresis, and electrokinetics can be broadly used to achieve micro- and nano-scale particle separations. Dielectrophoresis (DEP) has been used for various manipulation, concentration, transport, and separation processes of biological particles owing to its early development, mature theory, low cost, and high throughput. Although numerous reviews have discussed the biological applications of DEP techniques, comprehensive descriptions of micro- and nano-scale particle separations feature less frequently in the literature. Therefore, this review summarizes the current state of particle separation attention to relevant technological developments and innovation, including theoretical simulation, microchannel structure, electrode material, pattern and its layout. Moreover, a brief overview of separation applications using DEP in combination with other technologies is also provided. Finally, conclusions, future guidelines, and suggestions for potential promotion are highlighted.
实现高水平的纯化和分离是必不可少的，这样诸如恶性细胞、有害细菌以及特殊蛋白质或生物分子等目标颗粒，才能满足药物分析、临床诊断、靶向治疗和食品防护中的高精度测量要求。此外，这还能揭示个体生物变异的内在本质和进化机制。因此，许多与光镊、微流体、声泳和电动学相关的技术可广泛用于实现微米和纳米级颗粒的分离。介电电泳（DEP）由于其发展较早、理论成熟、成本低且通量高，已被用于生物颗粒的各种操控、浓缩、运输和分离过程。尽管已有众多综述讨论了DEP技术的生物学应用，但关于微米和纳米级颗粒分离的全面描述在文献中较少见。因此，本综述总结了颗粒分离的现状，关注相关技术的发展与创新，包括理论模拟、微通道结构、电极材料、图案及其布局。此外，还简要概述了DEP与其他技术结合的分离应用。最后，突出了结论、未来指导方针以及潜在推广的建议。
