Larcombe Daniel E, Braes Robyn E, Croxford James T, Wilson James W, Figurski David H, Hoskisson Paul A
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK.
Villanova University, 800 Lancaster Avenue, Villanova, PA 19026, USA.
Access Microbiol. 2024 Jun 5;6(6). doi: 10.1099/acmi.0.000808.v3. eCollection 2024.
Conjugation of plasmids from is essential for the genetic manipulation of spp. To facilitate intergeneric conjugation from to the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.
质粒从……的接合对于……属细菌的基因操作至关重要。为了促进从……到……的属间接合，通常由非转移性辅助质粒pUZ8002提供基因转移所需的接合机制。在此，我们展示了pUZ8002的完整核苷酸序列，描述了此前未记录的构建过程，并提供了相对于亲本pUZ8质粒和另一个先前发表的pUZ8002序列的该序列细节。
