Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.
Institute of Crystallography, National Research Council, 00143 Rome, Italy.
Biosensors (Basel). 2024 Jul 16;14(7):344. doi: 10.3390/bios14070344.
Medical devices have progressed from their initial bulky forms to smart devices. However, their rigidity hampers their seamless integration into everyday life. The fields of stretchable, textile, and flexible electronics are emerging research areas with the potential to drive significant technological progress. This research presents a laboratory-based technique to produce highly sensitive and flexible biosensors for detecting the chikungunya virus. These biosensors are based on 0D nanomaterials and demonstrate significant advancements in voltammetry. The electrochemical platform was created utilizing the stencil printing (StPE) technique. Adapting the biosensor setup involved the selection of aptamer as the biorecognition element bound with silver nanoparticles (AgNPs). This biosensor was employed in the voltammetric identification of the Chikungunya virus antigen (CHIKV-Ag) within a solution containing 0.5 mM potassium ferro/ferri cyanide, a redox pair. The biosensor was employed to evaluate CHIKV-Ag within a human serum sample. It demonstrated a linear detection span ranging from 0.1 ng/mL to 1 μg/mL, with a detection limit of 0.1 ng/mL for CHIKV-Ag. The proposed approach, due to its flexibility in production and the electrocatalytic attributes displayed by the zero-dimensional nanostructure, presents innovative opportunities for cost-effective and tailored aptamer-based bioelectronics, thereby broadening the scope of this domain.
医疗器械已经从最初的笨重形式发展到智能设备。然而，它们的刚性阻碍了它们无缝融入日常生活。可拉伸、纺织和柔性电子领域是新兴的研究领域，有潜力推动重大技术进步。本研究提出了一种基于实验室的技术，用于生产用于检测基孔肯雅病毒的高灵敏度和灵活的生物传感器。这些生物传感器基于 0D 纳米材料，并在伏安法方面取得了重大进展。电化学平台是利用模板印刷（StPE）技术创建的。适应生物传感器设置涉及选择适体作为与银纳米颗粒（AgNPs）结合的生物识别元件。该生物传感器用于在含有 0.5mM 铁氰化钾/亚铁氰化钾的溶液中对基孔肯雅病毒抗原（CHIKV-Ag）进行伏安识别，这是一对氧化还原对。该生物传感器用于评估人血清样本中的 CHIKV-Ag。它表现出从 0.1ng/mL 到 1μg/mL 的线性检测范围，CHIKV-Ag 的检测限为 0.1ng/mL。由于其生产的灵活性和零维纳米结构显示的电催化特性，该方法为基于适配体的经济高效的生物电子学提供了创新机会，从而扩大了该领域的范围。
