Terranova V P, DiFlorio R, Lyall R M, Hic S, Friesel R, Maciag T
J Cell Biol. 1985 Dec;101(6):2330-4. doi: 10.1083/jcb.101.6.2330.
The response of human endothelial cell migration to various extracellular matrix components and growth factors has been assessed. Human endothelial cells demonstrate increased chemotaxis and chemokinesis when placed in a modified Boyden chamber with endothelial cell growth factor (ECGF) used at a concentration of 10(-9) M. Anti-ECGF antibody inhibits the chemotactic response. Heparin (10(-8) to 10(-10) M) was also chemotactic and was shown to potentiate the chemotactic activity of ECGF. Although laminin, fibronectin, the polypeptide (epidermal, fibroblast, and nerve) growth factors, and collagen types I, II, III, IV, and V demonstrate a chemotactic response, these activities were one third to one half less than observed with ECGF. These data suggest that ECGF and heparin may play a significant role as response modifiers of human endothelial cell migration which may be relevant to tumor metastasis, wound healing, and atherogenesis.
已评估了人类内皮细胞迁移对各种细胞外基质成分和生长因子的反应。当将人类内皮细胞置于改良的博伊登小室中，并使用浓度为10(-9)M的内皮细胞生长因子（ECGF）时，细胞表现出趋化性和化学增活作用增强。抗ECGF抗体可抑制趋化反应。肝素（10(-8)至10(-10)M）也具有趋化性，并显示可增强ECGF的趋化活性。尽管层粘连蛋白、纤连蛋白、多肽（表皮、成纤维细胞和神经）生长因子以及I、II、III、IV和V型胶原表现出趋化反应，但这些活性比ECGF观察到的低三分之一至二分之一。这些数据表明，ECGF和肝素可能作为人类内皮细胞迁移的反应调节因子发挥重要作用，这可能与肿瘤转移、伤口愈合和动脉粥样硬化的发生有关。
