Rosales Saray, Hernández-Gutiérrez Rodolfo, Oaxaca Alma, López Zaira, Casillas Norberto, Knauth Peter, Quintero Luis H, Paz José A, Cholico Francisco, Velásquez Celso, Cano Mario E
Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragan 1421, Guadalajara 44430, Jalisco, Mexico.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara 44270, Jalisco, Mexico.
Bioengineering (Basel). 2024 Jun 21;11(7):638. doi: 10.3390/bioengineering11070638.
In this work, the cell line SW620-GFP has been used in a complete magnetic hyperthermia assay, from the preparation of the ferrofluid with folate-coated iron oxide nanoparticles to in vivo experiments. The physical and chemical characterization of the nanoparticles evidenced their superparamagnetic behaviour, an average diameter of 12 ± 4 nm, a 2 nm coat thickness, and a high-power loss density. The main innovation of the work is the exclusive capability of viable SW620-GFP cells to emit fluorescence, enabling fast analysis of both, cell viability in vitro with an epifluorescence microscope and tumour size and shape in vivo in a non-invasive manner using the iBox technology. Moreover, with this imaging technique, it was possible to demonstrate the successful tumour size reduction in mice applying magnetic hyperthermia three times a week over 3 weeks.
在这项工作中，细胞系SW620-GFP已被用于完整的磁热疗分析，从用叶酸包被的氧化铁纳米颗粒制备铁流体到体内实验。纳米颗粒的物理和化学表征证明了它们的超顺磁行为、平均直径为12±4纳米、涂层厚度为2纳米以及高功率损耗密度。这项工作的主要创新之处在于活的SW620-GFP细胞具有独特的发射荧光的能力，这使得使用落射荧光显微镜能够在体外快速分析细胞活力，并且使用iBox技术能够以非侵入性方式在体内分析肿瘤大小和形状。此外，通过这种成像技术，有可能证明在3周内每周对小鼠进行三次磁热疗后肿瘤大小成功减小。
