Cortés-Llanos Belén, Ocampo Sandra M, de la Cueva Leonor, Calvo Gabriel F, Belmonte-Beitia Juan, Pérez Lucas, Salas Gorka, Ayuso-Sacido Ángel
IMDEA Nanoscience, Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain.
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Nanomaterials (Basel). 2021 Oct 28;11(11):2888. doi: 10.3390/nano11112888.
Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules. The FBS concentration alters the labelling of all types of IONPs and hydrodynamic sizes ≥ 300 nm provide the greatest labelling using the centrifugation-mediated internalization (CMI). The best contrast for MRI results requires a core size range between 12-14 nm coated with dimercaptosuccinic acid (DMSA) producing values of 393.7 s and 428.3 s, respectively. These findings will help to bring IONPs as negative contrast agents into clinical settings.
氧化铁纳米颗粒（IONPs）是用于磁共振成像（MRI）中增强对比度的合适材料。它们潜在的临床应用范围从诊断到治疗以及后续治疗。然而，为了将该技术应用扩展到不同类型的癌症治疗，有必要更深入地了解IONPs、培养基和细胞之间的相互作用。为了获得这些相互作用的新见解，制备了一组具有相同无机核心和五种不同涂层的IONPs，以研究它们在不同生理介质中的聚集和相互作用，以及它们的细胞标记效率。然后，使用另一组具有六种不同核心尺寸和相同涂层的IONPs来研究核心尺寸如何影响体外细胞标记和MRI。在这里，悬浮在生物介质中的IONPs会经历部分涂层去除和分子粘附。胎牛血清（FBS）浓度会改变所有类型IONPs的标记情况，并且流体动力学尺寸≥300 nm的IONPs在通过离心介导的内化（CMI）时标记效果最佳。MRI结果的最佳对比度要求核心尺寸范围在12 - 14 nm之间，用二巯基琥珀酸（DMSA）包被，分别产生393.7 s和428.3 s的 值。这些发现将有助于将IONPs作为阴性造影剂引入临床应用。
