巴拿马皮肤利什曼病流行地区()物种分离株中RNA病毒的流行病学和基因特征
Epidemiology and Genetic Characterization of RNA Virus in () spp. Isolates from Cutaneous Leishmaniasis Endemic Areas in Panama.
作者信息
Bonilla Armando Assair, Pineda Vanessa, Calzada José Eduardo, Saldaña Azael, Laurenti Marcia Dalastra, Goya Stephanie, Abrego Leyda, González Kadir
机构信息
Programa de Maestría en Ciencias Parasitológicas, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Panama 3366, Panama.
Departamento de Investigación en Parasitología, Instituto de Conmemorativo Gorgas de Estudios de la Salud, Panama 0816-02593, Panama.
出版信息
Microorganisms. 2024 Jun 27;12(7):1317. doi: 10.3390/microorganisms12071317.
() spp. can harbor a double-stranded RNA virus known as Leishmania RNA virus 1 (LRV-1), whose presence has been reported in nine countries across the Americas and seven species. Here, we studied 100 () isolates from patients with cutaneous leishmaniasis collected from different endemic areas in Panama from 2016 to 2022. We identified () , () , () hybrid, and () sp.1. (genetic variant). LRV-1 was detected by RT-PCR in 9% of isolates (eight cases in () , and one in () . Phylogenetic analysis based on sequencing data classified all LRV-1 isolates within genotype A, suggesting that LRV phylogenetic proximity is closely aligned with geographical distribution or to the phylogenetic proximity of the host in the case of the () and () in Panama.
（利什曼原虫属）某些物种可携带一种名为利什曼原虫RNA病毒1（LRV - 1）的双链RNA病毒，在美洲的9个国家和7个物种中都有该病毒存在的报道。在此，我们研究了2016年至2022年从巴拿马不同流行地区收集的100株来自皮肤利什曼病患者的（利什曼原虫属）分离株。我们鉴定出了（某种利什曼原虫）、（另一种利什曼原虫）、（杂交种）和（利什曼原虫属1.（基因变体））。通过逆转录聚合酶链反应（RT - PCR）在9%的分离株中检测到了LRV - 1（在（某种利什曼原虫）中有8例，在（另一种利什曼原虫）中有1例）。基于测序数据的系统发育分析将所有LRV - 1分离株归类为A基因型，这表明在巴拿马，LRV的系统发育亲缘关系与地理分布密切相关，或者在（某种利什曼原虫）和（另一种利什曼原虫）的情况下与宿主的系统发育亲缘关系密切相关。
Zotero 插件