Van Poucke L S
Laboratory of Pharmaceutical Microbiology and Hygiene, State University of Ghent, Belgium.
Appl Environ Microbiol. 1990 Apr;56(4):924-7. doi: 10.1128/aem.56.4.924-927.1990.
A micro-enzyme-linked immunosorbent assay (micro-ELISA) using the Salmonella-TEK screen kit was tested for the detection of Salmonella spp. in pure cultures as well as in 30 artificially contaminated food samples and in 45 naturally contaminated food samples. Different raw, fleshy foods and processed foods were used as test products. The artificially contaminated minced meat samples were preenriched in buffered peptone water, and after incubation, different selective enrichment broths were tested. The micro-ELISA optical density values after enrichment and isolation of the different broths were very analogous. The quickest method to detect Salmonella spp. in different foods is to enrich them with Salmosyst broth, which reduces the total analysis time to 31 h. The Salmonella-TEK kit for Salmonella spp. provides a promising test for the detection of Salmonella antigens in food even when they are present at a low concentration (1 to 5 CFU/25 g). The cross-reaction of the anti-Salmonella antibodies, especially to other gram-negative bacteria, is nil.
使用沙门氏菌-TEK筛查试剂盒的微量酶联免疫吸附测定法（微量ELISA），被用于检测纯培养物、30份人工污染食品样本以及45份天然污染食品样本中的沙门氏菌属。不同的生鲜肉质食品和加工食品被用作测试产品。人工污染的碎肉样本先在缓冲蛋白胨水中进行预富集，培养后，对不同的选择性富集肉汤进行测试。不同肉汤富集和分离后的微量ELISA光密度值非常相似。检测不同食品中沙门氏菌属的最快方法是用Salmosyst肉汤进行富集，这将总分析时间缩短至31小时。用于沙门氏菌属的沙门氏菌-TEK试剂盒，即使食品中沙门氏菌抗原浓度较低（1至5 CFU/25 g）时，也能为检测提供有前景的测试。抗沙门氏菌抗体，尤其是与其他革兰氏阴性菌的交叉反应为零。
