A simple ELISA for antigiardial antibody using the membrane fraction of the parasite as antigen.
作者信息
Mohimen A, Maitra T K, Jalan K N, Dhar A
出版信息
Asian Pac J Allergy Immunol. 1985 Dec;3(2):183-6.
PMID:3907652
Abstract
Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.
摘要
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