State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China.
Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China.
Bioorg Chem. 2024 Oct;151:107692. doi: 10.1016/j.bioorg.2024.107692. Epub 2024 Aug 4.
As one of a traditional Chinese medicine with dual applications in both medicinal treatment and dietary consumption, the mature seeds of D. lablab were reported to be rich in saponins and have a good effect on inflammatory related diseases. However, the substance basis for its anti-inflammatory activity remains unclear. Thus, a comprehensive phytochemical investigation on triterpenoid saponins from D. lablab seeds was carried out, resulting in the isolation and identification of twenty-one new triterpenoid saponins including dolilabsaponins A-A, B, C, D-D, E-M, N, N and O (1-21) along with thirteen known analogs (22-34). Notably, the known saponins, 31, 32, and 34 were obtained from Leguminosae family for the first time. The H and C NMR data of saponins 24 and 28 were firstly reported here. Additionally, lipopolysaccharide (LPS)-stimulated RAW264.7 cells model was utilized to assess inhibitory activities of compounds 1-34 on nitric oxide (NO) production. The results revealed that compounds 1-3, 9, 10, 13-15, 18, 22, 23 and 28-34 significantly suppressed the elevation of NO levels in LPS-induced RAW264.7 cells at the concentration of 30 μM, exhibiting a concentration-dependent manner at 3, 10, and 30 μM. The results suggested that compounds 1-3, 9, 10, 13-15, 18, 22, 23, and 28-34 possessed potential anti-inflammatory activity. Further western blot assay demonstrated that 1, 9, 10, 13, 14, and 18 suppressed inflammatory response via down-regulated the expression levels of inflammatory factors, tumor necrosis factor-alpha and interleukin-6.
作为一种兼具药用和食用双重功效的中药，成熟的豇豆种子被报道富含皂苷，对炎症相关疾病有很好的疗效。然而，其抗炎活性的物质基础尚不清楚。因此，对豇豆种子中的三萜皂苷进行了全面的植物化学研究，分离并鉴定了 21 种新的三萜皂苷，包括 Dolilabsaponins A-A、B、C、D-D、E-M、N、N 和 O（1-21）以及 13 种已知类似物（22-34）。值得注意的是，首次从豆科植物中获得了已知皂苷 31、32 和 34。此外，还首次报道了皂苷 24 和 28 的 H 和 C NMR 数据。此外，利用脂多糖（LPS）刺激的 RAW264.7 细胞模型评估了化合物 1-34 对一氧化氮（NO）产生的抑制活性。结果表明，化合物 1-3、9、10、13-15、18、22、23 和 28-34 在 30 μM 浓度下显著抑制 LPS 诱导的 RAW264.7 细胞中 NO 水平的升高，在 3、10 和 30 μM 时呈浓度依赖性。结果表明，化合物 1-3、9、10、13-15、18、22、23 和 28-34 具有潜在的抗炎活性。进一步的 Western blot 检测表明，化合物 1、9、10、13、14 和 18 通过下调炎症因子肿瘤坏死因子-α和白细胞介素-6 的表达水平抑制炎症反应。
