Kannan Neha, S Gheena, Ramani Pratibha, Shanmugam Rajeshkumar, Ramalingam Karthikeyan
Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Nanobiomedicine Lab, Centre for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Jul 6;16(7):e63940. doi: 10.7759/cureus.63940. eCollection 2024 Jul.
The clinical use of antimicrobial agents for managing aphthous ulcers and periodontal diseases has long been a subject of intensive research by numerous investigators. As concerns over the side effects and antibiotic resistance associated with conventional therapies persist, there has been a concerted effort to explore alternative medicinal approaches. In line with this objective, our study introduces a novel herbal gum paint designed specifically to address the therapeutic needs of individuals suffering from oral ulcers and periodontal diseases.
The herbal formulation utilized in our study was prepared using extracts derived from Licorice () and Triphala, a combination of three fruits: , , and . These ingredients were selected based on their documented medicinal properties. The preparation process involved extraction and formulation techniques optimized for maximum efficacy. Antimicrobial activity was assessed using the bacterial culture method, where the formulation's ability to inhibit the growth of specific bacterial strains relevant to oral health was tested. Meanwhile, cytotoxicity was evaluated using the Brine Shrimp Assay method. Statistical analysis was conducted using a one-way analysis of variance (ANOVA) and Tukey post hoc test to validate the significance of our findings with statistical significance set at p<0.05.
The formulation exhibited significant activity against microbes when compared to the control. The cytotoxic activity was present at a concentration of 60 and 80µL, which indicated safe usage within specified concentration ranges, highlighting its potential for clinical application without adverse effects on biological systems. Statistically significant differences were obtained between the antimicrobial activity of the formulated gum paint and the commercial gum paint against species at 25 µL and 80 µL (p=0.00).
The study underscores the promising therapeutic potential of the herbal gum paint developed in this research. By harnessing the natural antimicrobial and anti-inflammatory properties of Licorice and Triphala, the formulated gum paint showed efficacy against These findings contribute to the growing body of evidence supporting the integration of herbal remedies into mainstream oral healthcare practices. Future investigations could further elucidate the mechanisms underlying its therapeutic actions and explore its broader clinical applications in diverse patient populations.
抗菌药物在治疗复发性口腔溃疡和牙周疾病方面的临床应用长期以来一直是众多研究人员深入研究的课题。由于对传统疗法的副作用和抗生素耐药性的担忧持续存在，人们一致努力探索替代医学方法。为了实现这一目标，我们的研究推出了一种新型草药牙龈涂料，专门用于满足口腔溃疡和牙周疾病患者的治疗需求。
我们研究中使用的草药配方是用甘草提取物和三果木（由三种果实：诃子、毗黎勒和余甘子组成）制备的。这些成分是根据其已记录的药用特性选择的。制备过程涉及为实现最大功效而优化的提取和配方技术。使用细菌培养法评估抗菌活性，测试该配方抑制与口腔健康相关的特定细菌菌株生长的能力。同时，使用卤虫试验法评估细胞毒性。使用单因素方差分析（ANOVA）和Tukey事后检验进行统计分析，以验证我们的研究结果的显著性，设定统计学显著性为p<0.05。
与对照组相比，该配方对微生物表现出显著活性。细胞毒性在浓度为60和80µL时出现，这表明在指定浓度范围内使用是安全的，突出了其在临床应用中的潜力，且对生物系统无不良影响。在25µL和80µL时，配方牙龈涂料与商业牙龈涂料对特定菌种的抗菌活性之间存在统计学显著差异（p=0.00）。
该研究强调了本研究中开发的草药牙龈涂料具有广阔的治疗潜力。通过利用甘草和三果木的天然抗菌和抗炎特性，制成的牙龈涂料对特定菌种显示出疗效。这些发现为支持将草药疗法纳入主流口腔保健实践的越来越多的证据做出了贡献。未来的研究可以进一步阐明其治疗作用的潜在机制，并探索其在不同患者群体中的更广泛临床应用。
