Department of Experimental Medicine, Histology and Embryology Section, University of Campania "L. Vanvitelli", Naples, Italy.
Methods Mol Biol. 2024;2835:39-48. doi: 10.1007/978-1-0716-3995-5_4.
Mitochondrial transfer (MT) is a biological process that allows a donor cell to horizontally share its own mitochondria with a recipient cell. Mitochondria are highly dynamic membrane-bound sub-cellular organelles prominently involved in the regulation of the cell energy balance, calcium homeostasis, and apoptotic machinery activation. They physiologically undergo fusion and fission processes in response to the cell requirement, with a continuous morphological re-arrangement. This structural and functional plasticity is at the basis of the MT, described in tissue regeneration, cardiac and neurological diseases, as well as in cancer. Here, the MT has been observed in the tumor micro-environment (TME) from the adipose-derived stem cells (ASCs) to the cancer cells, eventually reverting the lack of the mitochondria respiration function, or enhancing their motility and drug resistance. In this chapter, we outline some key protocols for evaluating this exciting phenomenon of MT. These methodological and technical approaches are very important, considering all the limitations that scientists constantly face, especially in this field of the research.
线粒体转移（MT）是一种生物学过程，允许供体细胞将自身的线粒体水平地与受体细胞共享。线粒体是高度动态的膜结合亚细胞细胞器，主要参与细胞能量平衡、钙稳态和凋亡机制的调节。它们在响应细胞需求时经历融合和裂变过程，不断进行形态学重新排列。这种结构和功能的可塑性是 MT 的基础，已在组织再生、心脏和神经疾病以及癌症中得到描述。在这里，已经观察到脂肪来源干细胞（ASCs）到癌细胞的肿瘤微环境（TME）中的 MT，最终恢复线粒体呼吸功能的缺失，或增强其运动性和耐药性。在本章中，我们概述了一些评估这种令人兴奋的 MT 现象的关键方案。这些方法学和技术方法非常重要，特别是考虑到科学家们在这个研究领域经常面临的所有限制。
