Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Telangana, India; Department of Chemistry and Biotechnology, Swinburne Institute of Technology, Victoria, Australia.
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Telangana, India.
Biomater Adv. 2022 Aug;139:212981. doi: 10.1016/j.bioadv.2022.212981. Epub 2022 Jun 14.
Stem cells based novel treatment modality for degenerative and immune dysfunction diseases created a huge demand of suitable carriers to support ex-vivo production of quality stem cells, and effective in-vivo transplantation of stem cells and their fate. In spite of promising candidature of nanofibrous microspheres (NFM) to recreate native stem cell niches to be used for possible scaling-up for ex-vivo stem cells expansion, it remains fairly unexplored. A systematic study on the stem cell-NFM interaction comparative with commercial microspheres (CM) has been performed for the first time. Gelatin NFM with variable physicochemical properties such as size, surface properties, surface chemistry, and variable degradability were prepared using microemulsion coupled with thermally induced phase separation (TIPS) method. Effect of physicochemical properties of NFM and their cellular interaction such as binding, morphology, metabolic activity and proliferation studies were performed using human bone marrow-derived mesenchymal stem cells (hBMSCs), human dental follicle stem cells (hDFSCs) and human gingival fibroblast (HGF) cells and compared with the commercial and solid microspheres. Gelatin NFM supports excellent cell binding, proliferation, metabolic activities and chemical cues specific differentiation. All out-turns indicate that NFM stand to be an outstanding candidate for ex-vivo cells' expansion and injectable carriers for stem cell transplantation.
基于干细胞的新型治疗方式为退行性和免疫功能障碍疾病创造了对合适载体的巨大需求，以支持体外生产高质量的干细胞，并实现干细胞的有效体内移植及其命运。尽管纳米纤维微球（NFM）有希望重现天然干细胞龛位，可用于体外干细胞扩增的规模化，但它仍未得到充分探索。首次对干细胞-NFM 相互作用与商业微球（CM）进行了系统研究。使用微乳液偶联热致相分离（TIPS）法制备了具有不同物理化学性质的明胶 NFM，如大小、表面性质、表面化学和可变降解性。使用人骨髓间充质干细胞（hBMSCs）、人牙囊干细胞（hDFSCs）和人牙龈成纤维细胞（HGF）细胞研究了 NFM 的物理化学性质及其细胞相互作用，如结合、形态、代谢活性和增殖，并与商业和固体微球进行了比较。明胶 NFM 支持优异的细胞结合、增殖、代谢活性和特定的化学分化。所有结果都表明，NFM 是体外细胞扩增和干细胞移植的理想注射载体。
