从患有X连锁B淋巴细胞缺陷的小鼠中产生抗III型肺炎球菌多糖杂交瘤。
Generation of anti-type III pneumococcal polysaccharide hybridomas from mice with an X-linked B-lymphocyte defect.
作者信息
Schroer K R, Kim K J, Prescott B, Baker P J
出版信息
J Exp Med. 1979 Sep 19;150(3):698-702. doi: 10.1084/jem.150.3.698.
Abstract
(CBA/N X BALB/c male)F1 mice bear on X-linked defect making them totally unresponsive to T-independent (TI), TI-2 antigens such as type III pneumococcal polysaccharide (SSS-III). We found that somatic cell hybrids between CB nonresponder spleen cells and NS1 plasmacytoma cells secreted antibody specific for SSS-III. The solid-phase binding of such antibody was completely inhibited by the addition of free antigen (SSS-III) and the amount of antibody detected in culture fluids ranged from 10 ng/ml to 10 micrograms/ml. Eight hybridoma clones were identified; all make antibody of the IgM class. These results indicate that the X-linked defect does not result in a deletion of a B-cell subset which responds to TI-2 antigens.
摘要
（CBA/N×BALB/c雄性）F1小鼠存在X连锁缺陷，使其对T细胞非依赖性（TI）、TI-2抗原（如III型肺炎球菌多糖（SSS-III））完全无反应。我们发现，CB无反应脾细胞与NS1浆细胞瘤细胞之间的体细胞杂交瘤分泌针对SSS-III的特异性抗体。加入游离抗原（SSS-III）可完全抑制此类抗体的固相结合，培养液中检测到的抗体量在10 ng/ml至10 μg/ml之间。鉴定出8个杂交瘤克隆；均产生IgM类抗体。这些结果表明，X连锁缺陷不会导致对TI-2抗原产生反应的B细胞亚群缺失。
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