Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, Germany.
Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743 Jena, Germany.
Chem Soc Rev. 2024 Sep 16;53(18):8957-8979. doi: 10.1039/d4cs00090k.
Surface enhanced Raman spectroscopy (SERS) is meeting the requirements in biomedical science being a highly sensitive and specific analytical tool. By employing portable Raman systems in combination with customized sample pre-treatment, point-of-care-testing (POCT) becomes feasible. Powerful SERS-active sensing surfaces with high stability and modification layers if required are available for testing and application in complex biological matrices such as body fluids, cells or tissues. This review summarizes the current state in sample collection and pretreatment in SERS detection protocols, SERS detection schemes, direct and indirect SERS as well as targeted and non-targeted SERS, and SERS-active sensing surfaces. Moreover, the recent developments and advances of SERS in biomedical application scenarios, such as infectious diseases, cancer diagnostics and therapeutic drug monitoring is given, which enables the readers to identify the sample collection and preparation protocols, SERS substrates and detection strategies that are best-suited for their specific applications in biomedicine.
表面增强拉曼光谱（SERS）作为一种高灵敏度和特异性的分析工具，满足了生物医学科学的要求。通过将便携式拉曼系统与定制的样品预处理相结合，实现了即时检测（POCT）。具有高稳定性和所需修饰层的强大 SERS 活性传感表面可用于测试和应用于复杂的生物基质，如体液、细胞或组织。本文综述了 SERS 检测方案中样品采集和预处理的现状，SERS 检测方案、直接和间接 SERS 以及靶向和非靶向 SERS，以及 SERS 活性传感表面。此外，还介绍了 SERS 在传染病、癌症诊断和治疗药物监测等生物医学应用场景中的最新发展和进展，使读者能够识别最适合其在生物医学中特定应用的样品采集和制备方案、SERS 基底和检测策略。
