用于测量单个细菌干物质和元素含量的X射线微分析法。
X-ray microanalytic method for measurement of dry matter and elemental content of individual bacteria.
作者信息
Heldal M, Norland S, Tumyr O
出版信息
Appl Environ Microbiol. 1985 Nov;50(5):1251-7. doi: 10.1128/aem.50.5.1251-1257.1985.
Abstract
A method for the determination of dry matter and elemental content of individual bacterial cells is described. The method is based on energy-dispersive X-ray microanalysis in a transmission electron microscope. A theory for area correction of intensity is developed. Escherichia coli in the late exponential phase of growth and early stationary phase (glucose limited) had an average dry matter content of 278 and 154 fg/cell, respectively. Of the elements detected, sodium, magnesium, phosphorus, sulphur, chlorine, potassium, and calcium together made up 15 to 17% of the dry matter content. A phosphorus content of 4.2 to 5.4% of the dry matter was found in these cells. Volume measurements of air-dried cells gave an average of 1.20 to 1.25 micron3. These results emphasize that dry matter content and elemental composition can be measured directly on single cells from complex microbial communities.
摘要
本文描述了一种测定单个细菌细胞干物质和元素含量的方法。该方法基于透射电子显微镜中的能量色散X射线微分析。建立了强度面积校正理论。处于生长指数后期和早期稳定期（葡萄糖受限）的大肠杆菌，其平均干物质含量分别为278和154 fg/细胞。在检测到的元素中，钠、镁、磷、硫、氯、钾和钙共占干物质含量的15%至17%。在这些细胞中发现磷含量占干物质的4.2%至5.4%。对风干细胞的体积测量结果平均为1.20至1.25立方微米。这些结果强调，可以直接对复杂微生物群落中的单个细胞进行干物质含量和元素组成的测量。
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