UMRt BioEcoAgro 1158-INRAE, BIOPI, Université de Picardie Jules Verne, 1 Rue des Louvels, 80000 Amiens, France.
Laboratoire Innovation Matériau Bois Habitat (LIMBHA), Ecole Supérieure du Bois, 7 Rue Christian Pauc, 44306 Nantes, France.
Molecules. 2024 Jul 26;29(15):3503. doi: 10.3390/molecules29153503.
Gabon has a rich flora, many species of which are used in traditional medicine. However, little research has been carried out on this wealth. An ethnopharmacological survey in the Fang language was carried out among traditional practitioners to collect antimicrobial medicinal plants. Phytochemical profiling of ethanolic and methanolic extracts from Mildbr leaves was carried out using HPLC-ESI-Q/TOF and a molecular network approach. Antibacterial activity was assessed with disk diffusion and microdilution methods, antioxidant activity via DPPH and FRAP methods, and in vitro cell viability via Cell Counting Kit-8. A total of 21 medicinal plants were collected, grouped into 10 families, of which the Fabaceae is the most represented. was chosen for chemical and biological studies due to its citation frequency (RCF = 0.59) and the absence of previous phytochemical studies. These studies revealed 4 major families of natural compounds and annotated 19 compounds for the first time. The crude leaf extract showed significant antioxidant and antibacterial activity. Cytotoxicity studies showed that the leaves were not cytotoxic, unlike the bark. This study underlines the importance of preserving the ancestral knowledge of the Fang populations, while showing promising results for .
加蓬拥有丰富的植物区系，其中许多物种被用于传统医学。然而，对这一财富的研究很少。在芳语中，对传统从业者进行了民族药理学调查，以收集抗菌药用植物。使用 HPLC-ESI-Q/TOF 和分子网络方法对 Mildbr 叶的醇提物和甲醇提取物进行了植物化学分析。采用圆盘扩散和微量稀释法评估了抗菌活性，采用 DPPH 和 FRAP 法评估了抗氧化活性，采用细胞计数试剂盒-8 法评估了体外细胞活力。共收集了 21 种药用植物，分为 10 科，其中豆科植物的种类最多。由于其引用频率（RCF = 0.59）和缺乏先前的植物化学研究，选择 Mildbr 进行化学和生物学研究。这些研究揭示了 4 类主要的天然化合物，并首次注释了 19 种化合物。粗提叶提取物表现出显著的抗氧化和抗菌活性。细胞毒性研究表明，与树皮不同，叶子没有细胞毒性。这项研究强调了保护芳族人群祖传知识的重要性，同时也为 Mildbr 叶显示出了有希望的结果。
